Gene Networks Database


Lytechinus variegatus Genes in Development: Primary mesenchyme-specific genes


LvSM50


Function

LvSM50 is a PMC marker gene. PMC-specific gene products participate in the synthesis of sceleton (Guss et al., 1997).
SM50 was previously characterized as a basic protein of the spicule matrix. The specific role of matrix proteins have not been elucidated, although they may function in the nucleation or orientation of crystal growth (Sucov et al., 1987; Livingston et al., 1991; Katoh-Fukui et al., 1991, 1992; Harkey et al., 1995).

Protein

Subcellular location


Expression Pattern

The LvSM50 transcript is approximately 1.6 kb in length. Although very faint signal is detectable at the hatched blastula stage by nothern blot analysis, levels increase significantly at the mesenchyme blastula stage. LvSM50 transcripts increase in abundance as development proceed.
By whole-mount in situ hybridization, LvSM50 transcripts are first detectable in PMCs at the mesenchyme blastula stage. This transcript is initially expressed at approximately equal levels in all PMCs but shows reproducible, regional variations in expression at later developmental stages. Specifically, a distinct increase in abundance is exhibited by PMCs in the scheitel by the late pluteus stage (Guss et al., 1997).

mRNA level

Temporal accumulation (persent maximum abundance*)

Method 1: Nothern blot hybridization
Reference: Guss et al., 1997

Stage
Hatched blastula
Early mesenchyme blastula
Late mesenchyme blastula
Midgastrula
Late gastrula
Prism
Early pluteus
Late pluteus
Level
0.1
2
20
30
27
37
41
100

*Values were corrected for relative amounts of hybridizable RNA based on ubiquitin expression levels.

Spatial localization

Method 1: Whole-mount in situ hybridization
Reference: Guss et al., 1997

Stage
Late mesenchyme blastula
Late gastrula
Pluteus larva
Tissue
all PMCs
all PMCs
all PMCs, PMCs in the scheitel (highest level)


Sequences


Regulatory Regions


Regulatory Connections

Upstream Genes

LvSM50

Downstream Genes


Evolutionary Homologues


Links


Bibliography


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