Gene Networks Database

Strongylocentrotus purpuratus Genes in Development: Transcription Factors



SpEts4 is a new member of the Ets transcription factor family.
Identification of SpEts4 suggests that SpEts4 and its closest relative Drosophila Ets4 constitute a new group within the Ets family (Wei et al., 1999).


The C-terminal amino acid region comprises a conserved domain of about 85 amino acids that is related to the DNA binding domain of the Ets family of transcription factors.
A second conserved sequence in the N-terminal region is called the pointed domain, its function is not well understood.
SpEts4 protein contains a transcription activation domain at position 36-123.
The activating region of SpEts4 is characterized by a relatively high density of residues that are also found in the activation domains of other transcription factors, which include glutamine, serine, proline, and acidic residues.
The sequences between amino acids 184 and 274 may be required for optimal activity of the upstream domain by providing either a coactivation or structural function (Wei et al., 1999).
GenBank: 4406284

Subcellular location

Expression Pattern

RNase protection assay demonstrated that SpEts4 transcripts are relatively abundant and present at nearly equivalent levels in the unfertilized egg and in embryos through the first six cleavages (8.5 h; 64-cell stage).
SpEts4 is also expressed at much later stages, after gastrulation is complete.
The timing of SpEts4 gene expression precedes the transient expression of SpHE in the very early sea urchin blastula (Wei et al., 1999).

mRNA level

Temporal accumulation

Method: RNase protection assay
Reference: Wei et al., 1999

3 hr cleavage
5.5 hr cleavage
8.5 hr cleavage
12 hr very early blastula
16 hr blastula
22 hr bkastula
36 hr gastrula
48 hr gastrula
72 hr pluteus
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Regulatory Regions

Regulatory Connections

Upstream Genes


Downstream Genes


Evolutionary Homologues



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