Gene Networks Database

LOCUS       IN_SPMTA       1120    DNA             INV       01-APR-1998
DEFINITION  Strongylocentrotus purpuratus metallothionein-A (MTA) gene intron 1
DBSOURCE    GENBANK:  name SUSMTA, accession M30606
KEYWORDS    metal-binding protein; metallothionein; metallothionein-A.
SOURCE      Strongylocentrotus purpuratus DNA.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Harlow,P., Watkins,E., Thornton,R.D. and Nemer,M.
  TITLE     Structure of an ectodermally expressed sea urchin metallothionein
            gene and characterization of its metal-responsive region
  JOURNAL   Mol. Cell. Biol. 9, 5445-5455 (1989)
  MEDLINE   93140799
 REFERENCE   3
   AUTHORS   Nemer,M., Stuebing,E.W., Bai,G., Parker,H.R.
   TITLE     Spatial regulation of SpMTA methallothionein gene expression
             in sea urchin embryos by a regulatory casette in intron 1
  JOURNAL    Mech. Dev. 50, 131-137 (1995)
   MEDLINE   95344989
 COMMENT     The proximal of 1.6 kb of the 5'-flanking region of SpMTA 
             metallothionein (MT) gene together with the 1.12 kb first intron 
             are sufficient for its correct cell-type specific expression in 
             transgenic embryos [2,3]. The heavy-methal ion-dependent activity of 
             promoter is sustained through the cooperative interaction of
             a series MRE sites [1]. The regulatory elements in intron enhance as
             well as restrict expression to the single set of embryonic cell
             lineages [2]. The restricted spatial expression is largely eliminated
             by the deletion of the interior 405-bp region in the intron.
             Within this region is 295-bp, genomically repetitive, 
             transposon-like segment [2,3], containing P3A site and four 
             apparent P5 sites, two of which are embeded in 
             25-bp inverted repeats at its termini. The P3A and terminal P5 sites 
             were shown [3] through competition to bind with relatively high
             affinities the same nuclear factors bound by their counterpart
             sites in the CyIIIa actin promoter
 FEATURES             Location/Qualifiers
      source          1..1120
                      /organism="Strongylocentrotus purpuratus"
                      /db_xref="taxon:7668"
                      /cell_type="sperm"
      repeat_region   256..261 
                      /note=matches 569..574; direct repeat
      misc_feature    265..559
		     /note=casette
                     /citation=[2]
      repeat_region   265..290 
                      /note=matches 544..568; inverted repeat
      protein_bind    272..286
                      /citation=[3]
                      /bound_moiety="P5 (SpTEF-1)"
                      /evidence=experimental
      misc_signal     307..316
                      /gene="MTA"
                      /standard_name="P7II"
                      /citation=[3]
                      /note="1 mismatch to consensus"
      misc_signal     368..383
                      /gene="MTA"
                      /standard_name="P5"
                      /citation=[3]
      protein_bind    417..425
                      /citation=[3]
                      /bound_moiety="SpP3A2"
                      /evidence=experimental
      misc_signal     510..524
                      /gene="MTA"
                      /standard_name="P5"
                      /citation=[3]
      protein_bind    546..561
                      /citation=[3]
                      /bound_moiety="P5 (SpTEF-1)"
                      /evidence=experimental
      repeat_region   544..568 
                      /note=matches 265..290; inverted repeat
      repeat_region   569..574
                      /note=matches 256..261; direct repeat
ORIGIN    
        1 gtaagcaaaa aatcggaatc aatgttctca ttaaaatacc gagttacaga ataccgtgta
       61 cgtacggtgt agcgtttcta gaaacaaacc gctcgatgtt acgtgcatac gatgatacga
      121 tacagccatg gccatgacca tgagaggccc ccgatcacga cacacgcagc tagctagacc
      181 gaattttccg tctctttcgt tcgttaagcc tcttatttgg gtagggtcgg ctctctcttt
      241 agagacggaa gccgactaga cctagggcgc gttcatgctt gattttgaag gcagaatgag
      301 cgatttgtaa cgctcatcgt aaacgctcat cgtaatcgca gttgcacggt gcactgttca
      361 tgctcacttc tccaggcaat tctccttggg tgccttcagc tggattcgca aacctgtgtg
      421 cgcatgctac gagagcccat agaggtcagt gcgttgaccc catttaaacg cgtttacaaa
      481 ttgtaaacgc atctttttgt gagtttacga tcggcgatca tgaatcggct ttttcggtga
      541 ttctacaaaa tcaagcatga acgcgcccct atactcattc ccttgctaac accaaggtgc
      601 atttattttt annnntatnc cnanaannnc cccaaatgtc attttacctc gtcgcatgtt
      661 atattgtaaa ttaggcctag gctataataa atcggttcca cttaccaact tgactgaaat
      721 aatttaatgg tcccttttta ggaaattatg ggtctgaaat cgaaatccat gttgaaaatc
      781 ttgcaaaacg tattttaact ctcgctgcgc acgacgtaat ctaaatctca tcccttctag
      841 tctacaccga aggcaaaatg attatgaagg aaaaactgtg cgcgagcttc acgttcgtta
      901 tcacagtttt tcttcataat cattttcatg ttttcttgcc cgtttcttca tacttgttac
      961 atcaacagtt atgtcaataa ctaagttttt accaacaagc actgctggat ttgtggtttc
     1021 aaattgcaag attgttatta cttttttaaa attttatatc attatataga ctatatatat
     1081 atatatatat atatattttt ttttttaatt ctgaacacag