Gene Networks Database

LOCUS       5FR_SPMTA       1699 bp    DNA             INV       17-JUN-1993
DEFINITION  Strongylocentrotus purpuratus metallothionein-A (MTA) gene, 5'-flanking region.
DBSOURCE    GENBANK:  name S80441, accession M30606
KEYWORDS    metal-binding protein; regulation; metallothionein-A.
SOURCE      Strongylocentrotus purpuratus DNA.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Harlow,P., Watkins,E., Thornton,R.D. and Nemer,M.
  TITLE     Structure of an ectodermally expressed sea urchin metallothionein
            gene and characterization of its metal-responsive region
  JOURNAL   Mol. Cell. Biol. 9, 5445-5455 (1989)
  MEDLINE   90066495
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Bai,G., Stuebing,E.W., Parker,H.R., Harlow,P. and Nemer,M.
  TITLE     Combinatorial regulation by promoter and intron 1 regions of the
            metallothionein SpMTA gene in the sea urchin embryo
  JOURNAL   Mol. Biol. Cell 13, 993-1001 (1993)
  MEDLINE   93140799
COMMENT     The proximal of 1.6 kb of the 5'-flanking region of SpMTA 
            metallothionein (MT) gene together with the 1.12 kb first intron 
            are sufficient for its correct cell-type specific expression in 
            transgenic embryos. The heavy-methal ion-dependent activity of 
            promoter is sustained through the cooperative interaction of
            a series MRE sites. The regulatory elements in intron enhance as
            well as restrict expression of the single set of embryonic cell
            lineages.
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /cell_type="sperm"
     misc_signal     1466..1473
                     /gene="MTA"
                     /standard_name="metal-responsive element (MREb)"
     misc_signal     1566..1573
                     /gene="MTA"
                     /standard_name="metal-responsive element (MREa)"
     TATA_signal     1592..1596
                     /gene="MTA"
     mRNA            1619..
                     /gene="MTA"
                     /number=1
     CDS             1697..
                     /gene="MTA"
                     /codon_start=1
                     /product="metallothionein-A"
                     /db_xref="PID:g161546"
ORIGIN      
        1 gatcctaagt cgactgaggt tcacggatca ggttgataaa cttgcagctc agccatggag
       61 gtatgcatca atccttgtat gataaaaaat gtactcgatg accccgtgaa gaacgaaggc
      121 ggcgggcaaa aatatacagg cattttaaga agtccagagc tacatgaaca tcttggttat
      181 aaatatatcc tgctgtggac tgcgaattga atgatgttta taatcctgtt tttacctttg
      241 gaaattgcag taataataga atgtaatacc tccaacattt ggtaaagagt ttggagttgg
      301 tatttcgtat gattattcag cgaattttag aaaacaagag cagttcaaaa atatttatcc
      361 atctagaagc cacacaagac cggtcacatg attcaaccca gccaaactca gaaaagctgc
      421 ccgcacataa gtgtactgta cgtgtcacgt atttaaataa tcttgaatat tcaaactatt
      481 gttatattat tcgtgtgcaa taatgaggac gagcatgcaa gcgctactcg agtgcaaacc
      541 caaccacctc tagtctctac ttaaggtgtt taaatcttta tctcaaagta tgacagaaaa
      601 gacgtcataa tcatatttgt atcacctccc ccctctcccc ttgggtaggg aaggcgaaat
      661 ttgagaaaac aatagaaatt gaacattaat aatgtattaa taatgtttat gctccatttc
      721 atgcatttcg tgtgaaacaa cgagttaaaa atatgcatgc agtaaatcct ttcgtctcca
      781 ctctgggtca atcgtcaggg gcagatccag gattcatttg aaatgaaatc gaggagggag
      841 gggggggggg gagatttcca ctgatgcctg gtgtgtagca ctatggcaat ttgactgggg
      901 gtaagtgtct ctaatacgcc ctataggcaa atattgacag gtctacgtaa tttagtatgg
      961 aaaaataacc attttatcat acaggttgat catcaatata tcattatgca gtgaacacca
     1021 tgaatatttc gaaatggagg gatagttgca tttctggcgg agatatccgg gtcgggaatc
     1081 atctactttt attccccctc cccatttttt tcttcttctc cttgttcttc ttctttttcg
     1141 tttcttaatc atggtattaa tcatttgcaa atcaattaca tgcctttata tatatataat
     1201 aatcatggtg ctacatgatt ttgaaatact ttatggaaac cttacgtttt accagacctt
     1261 gacacaaagt tgacagtgtt acctttatca ttcacttccg tactgtttca gcacatgagc
     1321 aacactttgt tctttgaggg caaacgtttt gggggagggg agggcatccc acggcgcttt
     1381 aaaaacaaaa aggatttttg cttcccttcc atatcccgta taatttatcc gacacgagcc
     1441 tagtcatcac cattcctgat agaagcgtgt gcaaaactag aaaggcccac ctagatcgag
     1501 ctcctcaggc accgctaaca ggttgcctag ctcgtgccta aacgtggtgt gtttacctat
     1561 ttgtttgcac acggatattg taatgaaaac atataaaacc ccgaatttca ccagtttttg
     1621 tcacaagctg aagcgtttga gtgtgattta cattaccttc tgaagaaaca tacaactcga
     1681 gttcaatttc atcaccatg
//