LOCUS SUSEGF2 1800 bp mRNA INV 18-MAR-1994
DEFINITION S.purpuratus epidermal growth factor-related protein (SpEGF2) mRNA,
complete cds.
ACCESSION M29004
NID g161463
KEYWORDS epidermal growth factor-related protein.
SOURCE Strongylocentrotus purpuratus embryo ectoderm cDNA to mRNA.
ORGANISM Strongylocentrotus purpuratus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
Strongylocentrotidae; Strongylocentrotus.
REFERENCE 1 (bases 1 to 1800)
AUTHORS Yang,O., Angerer,L.M. and Angerer,R.C.
TITLE Unusual pattern of accumulation of mRNA encoding an EGF-related
protein in sea urchin embryos
JOURNAL Science 246, 806-808 (1989)
MEDLINE 90049203
COMMENT Draft entry and computer-readable sequence for [Science (1989) In
press] kindly submitted
by R.C.Angerer, 13-OCT-1989.
FEATURES Location/Qualifiers
source 1..1800
/organism="Strongylocentrotus purpuratus"
/db_xref="taxon:7668"
/dev_stage="embryo"
/tissue_type="ectoderm"
gene 65..1658
/gene="EGF2"
CAAT_signal 65..69
/gene="EGF2"
repeat_region 170..179
/note="SpEGF2 direct repeat (glucocorticoid response
element)"
CAAT_signal 184..188
/gene="EGF2"
TATA_signal 206..211
/gene="EGF2"
repeat_region 250..259
/note="SpEGF2 direct repeat (glucocorticoid response
element)"
sig_peptide 308..358
/gene="EGF2"
CDS 308..1285
/gene="EGF2"
/note="precursor"
/codon_start=1
/product="epidermal growth factor-related protein"
/db_xref="PID:g161464"
/translation="MQFNLVFVVAVIGVGMVAADENLMSKLQMALKSMLNENEELQMQ
SRDTKGQCESDTNKCNNHGTCIEGRWGTYYCKCEMPFRVGIPDSSCYPPPEGKKDLEI
EARDSENRCLSDTSNCDGHGICQLSTFGRNERYICFCALGFRNNNYGGCSPYTPREIE
FLSYVARDLELEMLTRDSLGRCKSDTHNCDEAGQCVTKTYGRYAGEYICVCNHGYRNN
AYGGCSPMTTREIEYLDMLAREEQMQMLVRKYYSLSECSQGTNDCNENGECVEEDGKY
WCECGEGYEENEDGGCSPIVTRATEVDDDDFAERKKEIMRQLAEFLQEE"
mat_peptide 359..1282
/gene="EGF2"
/product="epidermal growth factor-related protein"
polyA_signal 1653..1658
/gene="EGF2"
BASE COUNT 511 a 392 c 430 g 467 t
ORIGIN
1 ttttaagatc tcaggtaatc gtgaaagaat gcagattaat acgaggaagc cttctacgca
61 aacgccaatt tctaaagact cgcagagttt aatgttcgcc cccggcccac cttgcaaaag
121 aaagtgtaat cggtggacgg atgagcacgc agtcgactgg gcgtggcact ttgtccttgc
181 gttccaattg ctccttgtgc acccctataa agagtgaccg gtcctgatga gcggggtagt
241 ttgttaaggt ttgtccttgg agaaagtact cttatgcttc gcactgtgtc aggattaaga
301 catcaagatg cagtttaacc ttgtttttgt ggtcgccgtc atcggcgtcg gaatggtagc
361 cgccgatgag aatctgatga gtaagctgca gatggctctg aaatcaatgc tcaatgaaaa
421 cgaggaactg caaatgcaaa gtcgcgacac caaggggcaa tgcgaatcag acaccaacaa
481 gtgcaacaac catggtacat gtattgaagg cagatgggga acgtactact gcaagtgtga
541 gatgccattt agggtgggca ttccagattc ttcttgctat ccacccccgg aagggaagaa
601 agacttggag attgaagccc gagacagtga gaacagatgc ttaagtgaca ccagcaactg
661 cgatggccat ggtatatgtc aactgagtac ctttggccgg aatgagcggt acatctgctt
721 ttgtgcactt ggattcagga acaacaacta cggcggttgc tctccctata caccccgcga
781 gatcgagttc ctgtcctacg ttgctcgcga cttagaactt gagatgctga ctcgtgacag
841 tttgggtagg tgcaaatcag atacccataa ctgtgacgag gctggtcagt gtgtgacgaa
901 aacctacgga cgatacgcag gagagtacat ttgcgtgtgc aatcacggct accggaacaa
961 cgcctacggc ggatgctcac ccatgacaac acgtgagatc gagtacctcg acatgcttgc
1021 ccgcgaggaa caaatgcaga tgctagtccg taaatactat tccttgtccg agtgcagcca
1081 aggaaccaac gactgcaacg aaaacggcga gtgtgtggag gaagacggaa agtactggtg
1141 cgagtgcgga gaaggctatg aggagaacga ggatggtgga tgctcaccca tagtaacacg
1201 tgccactgaa gtcgatgacg acgatttcgc tgaacgtaaa aaggaaatca tgcgacaact
1261 ggccgagttc ctccaagaag aataaaccat agcgtactaa gagagatagc tgtggcacga
1321 acgcgcctga agcggtcaaa ggtattatca tggtgcgtcg ttcccctacg gagaatgtgt
1381 gaggctccgt gaaatgactg gccaatcaca agcaacttcc agttacataa ctatctctgt
1441 tttgcgatgc ccgttttgga atcaagagca tgtagcttgc ttaattttcg ttttttgccc
1501 tcttcttctt cttctcctcc tccttggatt acttctttca ttttctttac cttaatgaaa
1561 cctttacatg attgttatca tgaattatgt tcatcataat gattatagag tacctttaat
1621 agcatttata aagcaccttt ctttcatttg atgtaaactt aaaatctgaa tatcaatatg
1681 cgatattaca agttgtagaa atgatagtct attcaatata atcttcaact tttgattatt
1741 ctagtcttcc agttgctgga ttttatcaaa ttaaacgacc tatcaaacaa aaaaaaaaaa
//