LOCUS PLHE6R 2880 bp RNA INV 05-MAY-1992
DEFINITION P. lividus mRNA for hatching enzyme.
ACCESSION X53598
NID g9995
KEYWORDS collagen homologue; Collagenase; metalloproteinase.
SOURCE common urchin.
ORGANISM Paracentrotus lividus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
Echinidae; Paracentrotus.
REFERENCE 1 (bases 1 to 2880)
AUTHORS Gache,C.
TITLE Direct Submission
JOURNAL Submitted (22-JUN-1990) C. Gache, URA 671 CNRS UNIVERSITY PARIS VI,
STATION ZOOLOGIQUE, 06230 VILLEFRANCHE SUR MER, FRANCE
REFERENCE 2 (bases 1 to 2880)
AUTHORS Lepage,T. and Gache,C.
TITLE Early expression of a collagenase-like hatching enzyme gene in the
sea urchin embryo
JOURNAL EMBO J. 9 (9), 3003-3012 (1990)
MEDLINE 90361016
FEATURES Location/Qualifiers
source 1..2880
/organism="Paracentrotus lividus"
/db_xref="taxon:7656"
/dev_stage="pre-hatching bastula"
/clone="HE6"
mRNA <1..2864
/gene="hatching enzyme"
gene 1..2864
/gene="hatching enzyme"
CDS 73..1836
/gene="hatching enzyme"
/codon_start=1
/product="pre-pro-hatching enzyme"
/db_xref="PID:g9996"
/db_xref="SWISS-PROT:P22757"
/translation="MANSGLILLVMFMIHVTTVHNVPLPSTAPSIITQLSDITTSIIE
EDAFGLTTPTTGLLTPVSENDSDDDGDDITTIQTTTSSSQTVISGVVVEEGVHESNVE
ILKAHLEKFGYTPPGSTFGEANLNYTSAILDFQEHGGINQTGILDADTAELLSTPRCG
VPDVLPFVTSSITWSRNQPVTYSFGALTSDLNQNDVKDEIRRAFRVWDDVSGLSFREV
PDTTSVDIRIKFGSYDHGDGISFDGRGGVLAHAFLPRNGDAHFDDSETWTEGTRSGTN
LFQVAAHEFGHSLGLYHSTVRSALMYPYYQGYVPNFRLDNDDIAGIRSLYGSNSGSGT
TTTTRRPTTTRATTTRRTTTTRATTTRATTTTTTSPSRPSPPRRACSGSFDAVVRDSS
NRIYALTGPYFWQLDQPSPSWGLVSNRFGFGLPQNIDASFQRGVVTYFFSECYYYYQT
STQRNFPRIPVNRKWVGLPCNIDAVYRSSRGPTYFFKDSFVYKFNSNNRLQRRTRISS
LFNDVPSALHDGVEAVVRADRNYIHFYRDGRYYRMTDYGRQFVNFPNGLPYSDVIESV
IPQCRGRSLSYESEGCSNSSE"
sig_peptide 73..126
/gene="hatching enzyme"
misc_feature 127..570
/gene="hatching enzyme"
/product="activation peptide"
mat_peptide 571..1833
/gene="hatching enzyme"
/product="hatching enzyme"
repeat_unit 1945..2472
/gene="hatching enzyme"
polyA_signal 2845..2850
/gene="hatching enzyme"
polyA_site 2864
/gene="hatching enzyme"
BASE COUNT 828 a 643 c 562 g 847 t
ORIGIN
1 ccaacaagat actcgactct cgaaaattac aaggttattt ttattcacga aataaatatc
61 gttggaaata aaatggcgaa ttctggcttg atattgctag tgatgttcat gatacatgtt
121 accactgtgc ataatgtccc gctacccagt acggcaccga gcatcatcac gcagttatct
181 gatattacga cgtcgatcat cgaggaggat gcttttggat tgacaacgcc gacaacaggt
241 ctactgactc ctgtctctga aaacgactcc gatgatgatg gtgacgacat tacaactata
301 caaacgacga catcatcatc gcagactgtg atttcgggcg tggtcgttga ggaaggcgtt
361 cacgaatcaa acgtcgaaat attaaaggca catctggaga agtttggcta caccccacct
421 ggttcgacct ttggcgaggc aaacctcaac tatacatcag ccatcttgga ttttcaagaa
481 catggcggca tcaaccaaac aggtatatta gacgcggata ctgctgaact tctttccacc
541 cctcgttgtg gtgttcctga tgtcttaccc tttgtgacgt caagcatcac gtggtctcgt
601 aatcaacctg ttacatacag ctttggcgca ctcacttccg atctaaacca gaatgatgtc
661 aaagacgaga taaggagagc attcagggtt tgggacgacg tctctggtct ttcattccgc
721 gaggtccctg atacgacatc ggttgatatc cgcatcaagt tcggcagcta cgaccacggc
781 gacgggatct ctttcgatgg ccgaggcggt gtactcgccc acgccttctt acctcgtaac
841 ggtgacgcgc attttgatga ctccgagacg tggacagaag gaactcgctc cggtactaac
901 ttgttccagg tagcagccca tgagtttggt cacagtctag gtctctatca ctccaccgta
961 cgatctgctc tcatgtaccc gtactaccag ggatacgtac ctaacttcag acttgacaat
1021 gacgacatcg ctggtatcag gtcactctat ggaagtaaca gcggcagtgg aactaccact
1081 acaaccaggc gaccgacaac cacccgtgcc accactacaa gacgaacaac caccacccgt
1141 gcaaccacta cccgtgccac caccaccacc accaccagcc cctctcgccc ttccccaccc
1201 cgacgggctt gctcaggatc ttttgatgcc gtcgttcggg atagtagcaa cagaatatac
1261 gctttgacgg gaccatactt ctggcagttg gatcaacctt caccttcatg gggtctagtt
1321 tctaaccgat ttggtttcgg cctccctcaa aatattgatg ccagcttcca aagaggagtc
1381 gttacctact tctttagtga atgttattac tactaccaga catcgactca acgtaacttc
1441 cctaggatac cagtcaatag gaaatgggtc ggtctaccat gtaatattga tgcagtgtac
1501 agatcatcaa gagggccaac ttacttcttc aaagattcat tcgtctacaa atttaacagc
1561 aacaaccggc tacaacggag aacgcgtatc tcgtctcttt tcaacgatgt acctagcgcc
1621 ctccacgatg gtgttgaagc agtcgtccga gctgacagaa attacattca cttctacagg
1681 gatggtcgtt actacaggat gacggactac gggagacagt ttgtcaactt tcccaatggt
1741 ttgccttata gtgacgtcat tgaatcagtc attccacagt gtcgcggacg gtctctgagt
1801 tacgagagcg aaggttgttc aaattcatcc gagtagatca tccgtgtagg gtataatctt
1861 taaaataacg ttatttattt tgtaagtaac agcatcagaa tagatcatcc gtctgcgcgg
1921 tataatcttt taataacttt tttttaaagg agaattgtcc ctttgtggat agttgactta
1981 ctgtggatgc agaaaaatca aataagcaca ttggtgaaga tttggtgaaa atcggataaa
2041 tgataagaaa gttatgagta tttgtatatt gagatctcta atgacatagg aaatctccca
2101 ttggcaacgc ggtcaccatt tgtgatgtca caaagggaga actcctcata tggtgtgcac
2161 aaaaactatt caaaacgtct ctttattttc attctaatga tagaacagct tttcttttca
2221 tgatatatta tgactttcat atatttacaa gtcatcccat aaaaagattc tttattcttt
2281 atagatatgg gaaagatctc cctgcattac atcacacatc atggtcgtgt tgccaattgg
2341 aggatatcat aagtgatctc aaaatctaaa aattcatatc tctcttattc atttcctgat
2401 ttttctgaaa ctttcccaat atgttttgag gctttgtctc cattcactga agctaaattt
2461 ctctgagggg ataattcccc tttaaatacc attgtttaac aaattttaca aagatgaaag
2521 tttgttcgaa ttcatcagaa tcaatcatcc agtggtttaa tcttcaagaa aatattgttt
2581 gataattagt gagcgaatta attgtaattc tttctatgca tttaataatt gtatgtcatt
2641 tggtctctgt atcacatttc aggagataca tttaatgctt gaaatgcatg aagtatagga
2701 cacatcaata gtatttcata acaagttagg tcagattgtg atttgcttta tacttaacaa
2761 taatttgtaa tttgtaatac ttcaatattt attatgtctt taatattgtt atttataggt
2821 attcctgtgg aattactgtt tgtaaataaa ttcatgaaca ccagaaaaaa aaaaaaaaaa
//