LOCUS       SPSPE2C4      141 bp    DNA             INV       19-JUN-1992
DEFINITION  Strongylocentrotus purpuratus Spec2c gene exon 4.
NID         g10300
KEYWORDS    calcium binding protein; Spec2 gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 141)
  AUTHORS   Hardin,P.E., Angerer,L.M., Hardin,S.H., Angerer,R.C. and Klein,W.H.
  TITLE     Spec2 genes of Strongylocentrotus purpuratus. Structure and
            differential expression in embryonic aboral ectoderm cells
  JOURNAL   J. Mol. Biol. 202 (3), 417-431 (1988)
  MEDLINE   89011969
COMMENT     see x12772, x14534 - x14538 for Spec2C gene exon seq. Data kindly
            reviewed (09-MAY-1989) by Klein W.H.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Strongylocentrotus purpuratus"
                     /clone_lib="lambda EMBL3."
     intron          <1..14
                     /note="Intron III (approx. 4.7 kb)"
     precursor_RNA   <1..>141
                     /note="primary transcript"
     CDS             15..135
                     /note="Spec2c protein (AA 62 - 102) (15 is 2nd base in
                     codon) (135 is 2nd base in codon)"
     mRNA            15..135
                     /note="Exon 4"
     intron          136..>141
                     /note="Intron IV (approx. 0.8 kb)"
BASE COUNT       43 a     27 c     38 g     32 t      1 others
        1 cttnattcgg ctaggtagca gacctgtaga cttttctgag attttgatga agaaggcaga
       61 acagatgcgc gggaaaggag cacaatactt taaagctttc gacgccttgg acacggataa
      121 aagcggatcc ctcaggtaag a