The Non-Agglutinating Phenotype Likely Results from an incomplete Glycosylation and/ or Mistargeted Delivery of Agglutinin to the Flagellar Surface. In wild-type cells (left panel), the agglutinins are likely processed in the Golgi, trans-Golgi network, and/or endosomal compartments, areas of low pH generated by proton pumps. From these compartments, the agglutinins are targeted to the flagella. In the mutant strain described here (right panel), the proton motive ATPase is absent so a low pH is not maintained in the appropriate compartments. The loss of agglutination in this mutant, therefore, likely results from an incomplete glycosylation and/or mistargeted delivery of the agglutinin.

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