RNA Analysis


A 4400 Nucleotide Message is not Expressed in the Mutant. Genomic DNA adjacent to the site of insertion was used to probe an RNA blot of total RNA isolated from mt+ and mt- gametes and vegetative cells, mutant gametes and vegetative cells, and from wild-type zygotes. A 4400 nt message was expressed at relatively high levels in the wild-type vegetative cells and zygotes, but expression was greatly reduced in wild-type gametes. The message was completely absent from the mutant cells indicating that the integration event generated a null mutation.

Previous Abstract Next