- Mutant strain fails to agglutinate with mt+
- RNA analysis indicates the mutant strain
lacks a 4.4 kB
mRNA normally expressed in vegetative cells and gametes of both
- Sequence analysis suggests that the defective
gene displays a stong homology to a H+ -ATPase genes.
- Constructs A and B will be used for complementation
- Western blot of microsomal membrane proteins
with H+-ATPase antibody develped against Arabidopsis H+
- Immunoflourescence of H+ -ATPase in wild-typeChlamydomonas.