Bio111: Molecules, Genes and Cells Lab Syllabus Spring 2009
Prof Bernd

Date Topic Pages
Jan 14-15 Introduction to the lab; safety issues; using a Pipetman:
 Introduction to spectrophotometry standard curves  		LM1-16
Jan 21-22 No Lab  
Jan 28-29 Enzymes: What are they? How do we study them? What parameters affect their function?Isocitrate Dehydrogenase (IDH) as example enzyme LM17-35
Feb 4-5 Parameters that Affect IDH Activity: Independent Experiments LM36-44

Feb 11-12

 Data Analysis and In lab Writing workshop: Experimental procedures and results sections
Consider all of your audience (make sure your figures are accessible to the colorblind)
Suggestions for appropriate figure construction
Departmental statement on plagiarism

LM45-55
grading rubric
Feb 18-19

DUE: 1) Oral presentation of Enzyme study (grading rubrics: make sure yours measures up)
2) Lab article: Title, Experimental procedures, Results (w/ figures) sections
New unit: Molecular biology and genetics: Profiling your genome
What is your genotype? PCR: DNA extraction and reactions

 LM56-58 through D1S80 factoids
Feb 25-26

PCR results and analysis
Introduction to Chlamydomonas

 LM56-67
Mar 4-5 No Lab: Spring Break
  
Mar 11-12 Gene expression to function:
Protein production and localization studies: Building a Chlamydomonas Flagella		 LM68-77
Mar18-19 Redirect and refocus-- wrap up lecture material, time for Q and A (and perhaps a deep breath)
Bring your 'class notebook' (if it is different from your lab one)		  
Mar25-26 Parameters that Affect Flagellar Regeneration: Independent Experiments
 LM78-86
Apr1-2 Data Analysis and In lab Writing workshop: Writing an Introduction
Introduction to Ames test and discussion of potential mutagens		 LM87-96
Apr 8-9

Due: 1) Oral Presentations
2) Lab Article: Title, Introduction, Results (w/ figures) and Ref sections of reports on Flagella Experiments
Bring YFPM to lab= Potential mutagen prep and planning

  
Apr 15-16 No Lab: Easter Break  
Apr 22-23 Spot-Overlay Ames Test of YFPM (Your Favorite Potential Mutagen) LM89-96
Apr 29-30 Quantifying a Mutagen LM97-101
  ARTICLES due by 5pm Wednesday (grading rubric)