Bio111: Molecules, Genes and Cells Prof Bernd
Lab Syllabus Spring 2011

Date Topic
Jan 13 Introduction to the lab; safety issues; using a Pipetman:
 Introduction to spectrophotometry and standard curves 
Jan 20 No Lab in observance of Martin Luther King Jr Holiday
Jan27 Enzymes: What are they? How do we study them? What parameters affect their function?Isocitrate Dehydrogenase (IDH) as example enzyme
Feb3 Parameters that Affect IDH Activity: Independent Experiments

Feb 10

NO Formal LAB: Covering data analysis material in class
Consider all of your audience (make sure your figures are accessible to the colorblind)
Suggestions for appropriate figure construction
Departmental statement on plagiarism
Lab space and computers available to work on figures with group

Feb 17

In lab writing workshop Learn to write-- and write Title, Abstract, Experimental procedures, and Results (w/ figures) sections --BRING figure drafts to lab
READ Pechenik
6th ed pd 151-198 (ch9), BRING Pechenik to lab
Grading rubric
New unit: Molecular biology and genetics: Profiling your genome

What is your genotype?
PCR: DNA extraction and reactions

Feb 24

PCR results and analysis
Introduction to Chlamydomonas
:
Microscopy basics

Mar 3 No Lab: Spring Break
Mar 10 Gene expression to function:
Protein production and localization studies: Building a Chlamydomonas Flagella
Mar 17 Parameters that Affect Flagellar Regeneration: Independent Experiments
Mar 24

BRING articles appropriate for Introduction (pdf or paper)
Data Analysis (stats) and In lab Writing workshop: Writing an Introduction
ReREAD Pechenik 166-198 READ Pechenik 139-150 (ch 8)
Introduction to Ames test and discussion of potential mutagens

Mar 31 Due: 1) Oral Presentations for Gene Expression / localization studies
2) Lab Article Due Parts: Title, Introduction, Results (w/ figures) and Ref sections of reports on Flagella Experiments Grading Rubric
Bring YFPM to lab= Potential mutagen prep and planning
Apr 7

Spot-Overlay Ames Test of YFPM (Your Favorite Potential Mutagen)

Apr 14 Quantifying a Mutagen (make sure you have read all of Pechenik Ch9)
Apr 21 ARTICLE DUE, course evaluations (grading rubric)
Apr 28

Available all day as extended office hours (no formal meeting)
Can also use lab as meeting place to work on pre-final questions in groups