Bio111: Molecules, Genes and Cells Prof Bernd Lab Syllabus Spring 2012

Date Topic
Jan 17 No Lab
Jan 24 Introduction to the lab; safety issues; using a Pipetman:
Spectrophotometry and standard curves COMPLETE LM6 & 7 questions and tables BEFORE lab
Jan 31 Enzymes: What are they? How do we study them? What parameters affect their function?Isocitrate Dehydrogenase (IDH) as example enzyme
Feb 7 Parameters that Affect IDH Activity: Independent Experiments
Feb 14 In lab writing workshop Learn about scientific conventions and then do it: Title, Abstract, Experimental procedures, and Results (w/ figures) sections
--BRING figure drafts to lab READ Pechenik 6th ed pd 151-198 (ch9), BRING Pechenik to lab
Consider all of your audience (make sure your figures are accessible to the colorblind)
Suggestions for appropriate figure construction
Departmental statement on plagiarism Read Grading rubric
Title, Abstract, Experimental Procedures and Results secion due by beginning of class on Wednesday.

Feb 21 New unit: Molecular biology and genetics:
Quantitative trait locus analysis:
Guest Instructor H. Cederholm, Duke University, Program in Cell and Molecular Biology Note this mini unit replaces the PCR genotyping laboratory in the lab manual.
Feb 28 New Unit: Gene expression to function: Protein production and localization studies
Introduction to Chlamydomonas and Microscopy basics
Mar 6 No Lab: Spring Break
Mar 13 Protein production and localization studiesBuilding a Chlamydomonas Flagella
Mar 20 Protein production and localization studies Parameters that Affect Flagellar Regeneration: Independent Experiments
Mar 27 Data Analysis (stats) and In lab Writing workshop: Writing an Introduction BRING articles appropriate for Introduction (pdf or paper)
ReREAD Pechenik 166-198 READ Pechenik 139-150 (ch 8)
Introduction to Ames test and discussion of potential mutagens
Apr 3 Due: 1) Oral Presentations for Gene Expression / localization studies
2) Lab Article Parts Due: Title, Introduction, Results (w/ figures) and Ref sections of reports on Flagella Experiments Grading Rubric
Bring YFPM to lab= Potential mutagen prep and planning
Apr 10 BREAK: No Lab
Apr 17 New Unit: Spot-Overlay Ames Test of YFPM (Your Favorite Potential Mutagen)
Apr 24 Quantifying a Mutagen (remember to return Thurs to count colonies)
May 1 FULL ARTICLE DUE (grading rubric), course evaluations