Bio111: Molecules, Genes and Cells Prof
Bernd Lab Syllabus Spring
2012
| Date | Topic |
| Jan 17 | No Lab |
| Jan 24 | Introduction to the
lab; safety issues; using a Pipetman: Spectrophotometry and standard curves COMPLETE LM6 & 7 questions and tables BEFORE lab |
| Jan 31 | Enzymes: What are they? How do we study them? What parameters affect their function?Isocitrate Dehydrogenase (IDH) as example enzyme |
| Feb 7 | Parameters that Affect IDH Activity: Independent Experiments |
| Feb 14 | In
lab writing workshop Learn about scientific conventions and then do it: Title, Abstract, Experimental procedures,
and Results (w/ figures) sections --BRING figure drafts to lab READ Pechenik 6th ed pd 151-198 (ch9), BRING Pechenik to lab Consider all of your audience (make sure your figures are accessible to the colorblind) Suggestions for appropriate figure construction Departmental statement on plagiarism Read Grading rubric Title, Abstract, Experimental Procedures and Results secion due by beginning of class on Wednesday. |
| Feb 21 | New unit: Molecular biology and genetics: Quantitative trait locus analysis: Guest Instructor H. Cederholm, Duke University, Program in Cell and Molecular Biology Note this mini unit replaces the PCR genotyping laboratory in the lab manual. |
| Feb 28 | New Unit: Gene
expression to function: Protein production and localization studies Introduction to Chlamydomonas and Microscopy basics |
| Mar 6 | No
Lab: Spring Break |
| Mar 13 | Protein production and localization studiesBuilding a Chlamydomonas Flagella |
| Mar 20 | Protein production and localization studies Parameters that Affect Flagellar Regeneration: Independent Experiments |
| Mar 27 | Data
Analysis (stats) and In lab Writing workshop: Writing an Introduction BRING articles appropriate
for Introduction (pdf or paper) ReREAD Pechenik 166-198 READ Pechenik 139-150 (ch 8) Introduction to Ames test and discussion of potential mutagens |
| Apr 3 | Due:
1) Oral Presentations for Gene Expression / localization studies 2) Lab Article Parts Due: Title, Introduction, Results (w/ figures) and Ref sections of reports on Flagella Experiments Grading Rubric Bring YFPM to lab= Potential mutagen prep and planning |
| Apr 10 | BREAK: No Lab |
| Apr 17 | New Unit: Spot-Overlay Ames Test of YFPM (Your Favorite Potential Mutagen) |
| Apr 24 | Quantifying a Mutagen (remember to return Thurs to count colonies) |
| May 1 | FULL ARTICLE DUE (grading rubric), course evaluations |