DNA Sequencing and RNA Detection Protocols for the Undergraduate Laboratory
|The development of these laboratory protocols
was accomplished through the hardwork of John McKillop under the supervision
of Karen Bernd, and Liz Nugent, Shannon Reidley, and Christine Larned under
the supervision of David Wessner. The project was funded through and Educational
Enhancement Grant from the North Carolina
Biotechnology Center, Durham, NC. Facilities and further support were
provided by Davidson College and the Davidson College Biology Department.
The protocols have been or are being course-tested
in upper level biology courses at Davidson College. DNA sequencing protocols
are being used in Microbiology (Bio302) to explore sequence conservation
and mutation rate in the hypervariable region of bacterial isocitrate dehydrogenase.
RNA isolation and detection methods have been implemented in Cell Biology
(Bio308) to characterize the effect of novel mating mutations on the expression
of components within the mating response signaling pathway. Manuscripts
describing these studies in detail are in preparation.
|© Copyright 2001 Department of Biology, Davidson College, Davidson,
NC 28036. Send comments, questions, and suggestions to: firstname.lastname@example.org