Introduction
DNA Sequencing
RNA Isolation & Detection
Acknowledgements

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

 

Return to Bernd Homepage

 
 
RNA Isolation and Detection Protocols for the Undergraduate Laboratory
Designed to study differential gene expression in S. cerevisiae during induction of the mating response. Methods optimized by John McKillop '01 for use in Karen Bernd's Cell Biology Laboratory; Bio308
Yeast mating as model for studying signaling pathways

 
 
Problems isolating RNA from yeast (and one solution)

 
 
 
 
 
 
 
 
 
 
 
 
Phenol Free RNA isolation

 
 
 
 
 
You got it--how good is it?

 
 
 
 
 
 
What's it good for?

 
 
 
 
 
Detecting specific RNA's



 
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