Step 1: Determine a potentially beneficial trait, and find the corresponding gene.

Step 2: Decode the protein of that gene and modify it to match the corresponding code of the DNA.

Step 3: Add the DNA code for a promoter gene and prepare a gene construct.

Step 4: Insert the gene construct into a bacterial plasmid.

Step 5: Insert the plasmid into a bacterial cell line, and allow the bacteria to multiply in order to acquire billions of copies of the desired plasmid.

Step 6: Isolate the plasmid from the bacteria and cleave it into linear cassettes.

Step 7: Inject a million or so cassettes into each newly fertilized, undivided egg of the fish to be modified; repeat hundreds of times.

Step 8: Incubate eggs and grow up surviving fry.

Step 9: The resulting fish that survive will vary greatly in the degree to which the added gene is expressed. Find the fish that are most like the desired result.

Step 10: Breed the fish to build a strong foundation in the genetic construction.

Step 11: Test the fish for food safety and seek FDA approval. Develop the data to show your transgenic fish strain is commercially desirable, safe to eat and safe when lost into the environment. Design biological or physical containment for the new variety of transgenic fish to protect wild populations from gene introgression and competition.

(Matheson, 1999)