Bio 363 Human Genetics Spring 2013
Transgenerational epigenetic inheritance study sheet
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Daxinger and Whitelaw, 2012:
What is the normal general pattern of genomic methylation during gametogenesis, fertilization, and embryogenesis?
What are examples of genes known to escape any element of this reprogramming pattern?
What's odd about the hepatitis B surface antigen receptor gene? (p. 2 second column near the bottom)
Summarize the various way the Avy allele of the agouti gene shows transgenerational epigenetic inheritance, including environmental effects (also see the Jirtle and Skinner review).
How can the glucocorticoid receptor gene show transgenerational epigenetic inheritance that is not via gametes? (also see Jirtle and Skinner)
What was shown to be a caveat in studies of the effects of vinclozolin?
What are the two categories of molecular mechanisms for transgenerational epigenetic inheritance, and what are some of the specific possible mechanisms in each category?

Jirtle and Skinner, 2007:
(In addition to questions mentioned above) What sorts of environmental factors can alter epigenetic regulation, and how?
Why are imprinted genes good candidates to be involved with transgenerational epigenetic inheritance?

Vassoler et al., 2013:
How exactly were experimental and control sire rats treated? What was done with their kids?
Figure 2: What was different about male and female offspring of treated vs control fathers?
Figure 3: What is a breakpoint? How does it show the differences in how desperate the male vs female offspring were for cocaine?
What important controls are only shown in supplementary figures?
Figure 4: What different types of experiments were done to get the data in each of the four panels? Are controls sufficient? How can there be more transcript when detecting exon IV than when detecting other exons?
Figure 5: How does this suggest the necessity of the BDNF role?
FIgure 6: How specific to BDNF are the data in panels a-f? What about g or h? How do the data in panel h correspond to the data in 4c?
What information regarding transcripts should have been in the discussion?
How does the histone/protamine situation provide a promising angle to help us understand paternal epigenetic transmisssion?

Manikkam et al., 2012:
What is dioxin? Look up examples of past public health situations related to dioxin.
How were the F0 rats treated? How were they outbred? Do we know if paternal or maternal transmission was followed? Which descendants were studied?
What were the most striking effects shown in Figures 1-4? Without looking at DNA methylation, would there be other possible explanations besides transgenerational epigenetic inheritance?
How were the data in Figure 1 collected? What internal control confirmed accuracy?
What categories of genes were affected? (Table 1, Figures 6-7)
Is there any concern that the same caveat for vinclozolin (see Daxinger and Whitelaw) might contribute here?
What would be helpful to know about dioxin?