BIO 111 - Molecules, Genes, and Cells
Dr. Haynes - Spring 2008

Lab Schedule

Section E meets every Monday, 1:30 pm - 4:20 pm
Section F meets every Wednesday, 1:30 pm - 4:20 pm
in Watson Life Sciences, Room 147

Part One: Methods Used for Cell Biology - Enzyme Measurements

Section E
Monday
Section F
Wednesday
Topics Pre-lab Reading
Jan 14 Jan 16
no lab
1. Safety, safety, safety!
2. How to use a Pipetman
3. Introduction to spectrophotometry
Study Guide: LM 1 - 15
Link: practice calculations
Jan 21
no lab
Jan 23 1. Safety
2. How to use a pipetman
3. Introduction to spectrophotometry
Study Guide: LM 1 - 15
Link: practice calculations
Jan 28 Jan 30 1. Measurement of isocitrate dehydrogenase (IDH) enzyme activity Study Guide: LM 17 - 35
Link: isocitrate dehydrogenase (IDH)
Feb 4 Feb 6 1. IDH physical models
2. Parameters that affect IDH activity
Study Guide: LM 35 - 44
Link: IDH dimer movie
Feb 11 Feb 13 1. Preparation for presentations and written reports Study Guide: LM 45 - 55
Link: Assignment Help Guide

Part Two: Methods Used for Genetics - PCR and Gel Electrophoresis

Section E
Monday
Section F
Wednesday
Topics Pre-lab Reading
Feb 18 Feb 20 1. Presentation 1: your IDH research
2. Turn in Written Report 1
3. Genotyping by PCR
Study Guide: LM 56 - 58
Links: Gel electrophoresis, PCR method
Feb 25 Feb 27 1. Analyzing PCR results
2. Using microscopes
3. Chlamydomonas flagella introduction
Study Guide: LM 58 - 62
Study Guide: LM 63 - 67
Link: The PCR Song
Mar 3 Mar 5 SPRING BREAK (no lab) none

Part Three: Methods Used for Biochemistry - Organelle Regeneration Kinetics

Section E
Monday
Section F
Wednesday
Topics Pre-lab Reading
Mar 10 Mar 12 1. Chlamydomonas flagellar regeneration Study Guide: LM 68 - 77
Mar 17 Mar 19 1. Conditions that affect flagellar regeneration Study Guide: LM 78 - 80
Mar 24 Mar 26
no lab
EASTER BREAK (no lab) none
Mar 31 Apr 2 1. Statistics with Excel
Study Guide: LM 81 - 86
Apr 7 Apr 9 1. Presentation 2: your Chlamy flagella research
2. Turn in Written Report 2
3. Prepping your favorite potential mutagen for the Ames test
Study Guide: LM 45 - 55, 87
Link: Assignment Help Guide

Part Four: Methods Used for Cancer Research - Mutagen Detection

Section E
Monday
Section F
Wednesday

Topics Pre-lab Reading
Apr 14 Apr 16 1. Pouring agar plates
2. Ames test - assay 1
Study Guide: LM 87, 89 - 96, 97 - 98, 88
Apr 21 Apr 23 1. Counting colonies
2. Quantifying a mutagen - assay 2 (count colonies on your own after 4 days of growth)
Study Guide: LM 98 - 101
Apr 28 Apr 30

1. Presentation 3: your mutagen research
2. Turn in Written Report 3

*Deadline for revised Written Report 3 is Wednesday May 7 (final exam day)*

End of lab - Have a Great Summer!

Study Guide: LM 49 - 54
Link: Assignment Help Guide

 

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