Fall 1999 Biology 111 Exam #2.5 - Molecular Genetics Half Exam

There is no time limit on this test, though I have tried to design one that you should be able to complete within 1.5 hours, except for typing. You are not allowed to use your notes, old tests, any electronic sources, any books, nor are you allowed to discuss the test with anyone until all exams are turned in by class on Monday November 1. EXAMS ARE DUE AT CLASS TIME ON MONDAY NOVEMBER 1. You may use a calculator and/or ruler. The answers to the questions must be typed on a separate sheet of paper unless the question specifically says to write the answer in the space provided. If you do not write your answers on the appropriate pages, I may not find them unless you have indicated where the answers are. There are 3 pages to this exam, including this cover sheet.


Please do not write or type your name on any page other than this cover page. Staple all your pages (INCLUDING THE TEST PAGES) together when finished with the exam.

Name (please print):


Write out the full pledge and sign:

Here is the honor code

"On my honor I have neither given nor received unauthorized information regarding this work, I have followed and will continue to observe all regulations regarding it, and I am unaware of any violation of the Honor Code by others."

How long did this exam take you to complete (excluding typing)?

3 pts.
1) Explain to me what a RFLP is and what the acronym stands for.

8 pts.
2) The RFLP used here is linked to the disease gene and is located 3.5 map units away.
a) Which band is associated with the disease allele?
b) Is this a dominant, recessive or codominant disease? How do you know?
c) What should you tell the expectant parents of the fetus labeled with a "?"? Be as specific as you can.

6 pts.
3) Construct a linkage map with these data that concern a disease locus and two RFLPs.

6 pts.
4) What components of DNA replication are used in DNA sequencing reactions and what components of sequencing reactions are unique to the sequencing reaction? You do NOT need to list components used in replication that are not used in sequencing.


8 pts.
5) Create a restriction map from these data generated with the two restriction enzymes, S and R. Draw the map on your typed pages.

5 pts.
6) Describe the genetic cause for most cases of cystic fibrosis. Be sure to include why this is a recessive disease at the molecular level.


6 pts.
7) a) Explain the molecular reason why Huntington's Disease is a dominant disease.
b) Could HD be treated with gene therapy the same way that CF can be treated?

8 pts.
8) Tell me the genotypes and phenotypes of all F2 children in this family that has a sex-linked disease caused by a metabolic disorder. Note that shading has been omitted from the F2 generation so to receive full credit, you MUST fill in the appropriate
shapes that HAVE TO BE shaded but do not fill in any shapes if you are not SURE that the child would have the disease. Do any shading on this page only.