A. Malcolm Campbell

Spotted Scanning Standards (S3)

Emily Oldham and Dan Pierce ('03)

Experimental Design

Need Stronger Signal

3DNA Dendrimer Advantage = 300+ dyes per binding





DNA Concentrations Printed



Establish Scanning Parameters



Concentrations Alters Ratios


Sequence-Specific Variations


Altered 3DNA Sticky Ends


Clean Up Printed DNA: Clone PCR Products



Plasmids Improve Signal



Microarrays Underestimate Fold Change



Biology Department

© Copyright 2004 Department of Biology, Davidson College, Davidson, NC 28036
Send comments, questions, and suggestions to: macampbell@davidson.edu