Reults from GCAT Undergraduates
Here are some sample data from Mary Lee Ledbetter's undergraduate lab at Holy Cross. These data were obtained with the same RNA sample, so all spots "should" be equal (i.e. yellow). Scans were conducted twice to obtain faint signals (long exposure) and to prevent saturation of the CCD camera (short exposure). The RNA was isolated from exponential phase cells grown aerobically on glucose. ISB slide number was 2828
Green Cy3 Tiff File
Red Cy5 Tiff File
Davidson College, NC (Dr. A. Malcolm Campbell, faculty advisor)
Pomona College, CA (Dr. Laura Hoopes, faculty advisor)
Missouri Western State College, MO (Dr. Todd Eckdahl, faculty advisor)
MWSC Student Presentations for 2000-2001 academic year (* denotes undergraduate authors):
1) Hansen*, Edward L. "Using Microarray Technology to Measure Effects of Minor Groove Binding Drugs on Gene Expression in Yeast." Oral Presentation at the Missouri Academy of Science, Collegiate Division, April 20, 2001, Missouri Southern State College, 2nd place award.
2) Eckdahl, Todd T, David Ash*, and Brianna Peterson*, "Using Microarray Technology in the Undergraduate Laboratory." Oral Presention at the Missouri Academy of Science, Senior Division, April 20, 2001, Missouri Southern State College.
3) Brown*, Abby. "Single Gene and Genome Wide Expression Analysis of Glucose and Galactose Metabolism in Saccharomyces cerevisae." Poster Presentation at the Beta Beta Beta NC-2 District Convention, April 28, 2001, Reis Biological Station.
4) Hansen*, Edward L. "Using Microarray Technology to Measure Effects of Minor Groove Binding Drugs on Gene Expression in Yeast." Oral Presentation at the Beta Beta Beta NC-2 District Convention, April 28, 2001, Reis Biological Station.
Muhlenberg College, PA (Dr. Denise Wallack, faculty advisor)
Swarthmore College, PA (Dr. Liz Vallen, faculty advisor)
Mount Saint Mary's College, MD (Dr. Myra Derbyshire, faculty advisor)
Microarray expression analysis of a Saccharomyces
cerevisiae hst3 hst4 double mutant
Dr. Myra K. Derbyshire 1, Daniel J. Koppers1,
Marlon G. Lawrence1, Heather L. Offutt1, Loretta
M. Rathvon1, Harriete L. Sanderson1, Nabil A., Shafi1,
Michael F. Smith1, Megan E. Walsh1, Dr. Jeffery
1Genetics class. Mount Saint Mary's College, Emmitsburg, Maryland.
2NCI-Frederick Cancer Research and Development Center, Frederick, Maryland.
The Saccharomyces cerevisiae SIR2 family
of genes consists of SIR2 and four SIR2-related genes (HST1-
HST4). The SIR2 gene product is a key regulator of gene expression
believed to help form modified chromatin structures on the genes it regulates.
SIR2 is important for the structure of the nucleolus, for repressing
gene expression near chromosome telomeres and at special sequences involved
in cell-type differentiation. HST1-HST4 gene products may modify
chromatin structure at different chromosomal locations from those regulated
by SIR2. S. cerevisiae hst3 hst4 double mutants have
low viability, defective telomeric silencing, and altered
cell-cycle progression. A hst3::kanMX4 (Saccharomyces Deletion Research Project, #11801) was crossed to a hst4::kanMX4 strain (#3350). The gene expression patterns of two meiotic segregants (HST3 HST4, hst3 hst4) were compared. Microarray technology was made possible through affiliation with GCAT (Genome Consortium for Active Teaching).
California Lutheran University, CA (Dr. Denis Revie, faculty advisor)
Lycoming College, PA (Dr. Jeff Newman, faculty advisor)
University of Southern Colorado (Dr. Dan Caprioglio, faculty advisor)
Carleton College, MN (Dr. Stephan Zweifel, faculty advisor)
Rice University, TX (Beth Beason, faculty)
GCAT members thank Drs. Pat Brown, David Botstein, Barbara Dunn, and the other members of the Stanford Microarray Database for their generosity in allowing undergraduate data to be included in their database. We also thank Pat Brown and members of his lab for producing the yeast microarrays for our use. Without this support, GCAT would not have been so successful in its first year of existence.
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