Genome Hybridization (CGH)
for Yeast DNA Microarrays
GH means you isolate total genomic DNA from two strains, label them different colors, and hybridize them to a normal yeast (that's what we use) DNA microarray. If the yeast has experience aneuploidy, then the genes that are duplicated or deleted will display green or red. Genes that are present at the same level in both strains will appear yellow. Below is a snapshot from an experiment that compared the same cell to itself. You can compare this result to those produced by published works at Stanford.
This protocol (PDF version; Word version) was developed by A. Jackie Ryan as a part of her honors research.
Data will be posted on the GCAT wiki.
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Department of Biology, Davidson College, Davidson, NC 28035
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