*This page is part of
undergraduate assignment for Molecular Biology at Davidson College
Cloning 5 Isoforms
of Isocitrate Dehydrogenase
IDH1:
Genebank Accession Number: NC_001146
DNA Sequence
Gene
Sequence (DNA with introns):
1 ATGCTTAACA GAACAATTGC TAAGAGAACT TTAGCCACTG CCGCTCAGGC 51 GGAACGCACC CTACCCAAGA AGTATGGCGG TCGTTTCACC GTCACTTTGA 101 TACCTGGTGA CGGTGTTGGG AAAGAAATCA CTGATTCAGT GAGAACCATT 151 TTTGAGGCTG AAAATATCCC GATCGACTGG GAAACTATAA ACATTAAGCA 201 AACAGATCAT AAGGAAGGCG TCTATGAAGC TGTTGAGTCT CTAAAGAGAA 251 ATAAGATTGG TCTTAAGGGG CTATGGCACA CTCCTGCTGA CCAAACAGGT 301 CACGGTTCAC TAAACGTTGC TTTGCGTAAA CAACTAGATA TCTACGCCAA 351 TGTGGCCCTT TTCAAATCCT TGAAGGGTGT CAAGACTAGA ATTCCAGACA 401 TAGATTTGAT TGTCATTAGA GAAAACACGG AGGGTGAGTT CTCAGGCCTG 451 GAACATGAAT CCGTCCCTGG TGTAGTGGAA TCTTTGAAAG TTATGACTAG 501 ACCTAAGACA GAAAGGATCG CCAGATTTGC CTTTGACTTC GCCAAGAAAT 551 ACAACAGAAA GTCTGTCACA GCTGTGCATA AGGCAAATAT CATGAAGTTA 601 GGTGACGGTC TGTTCAGAAA TATAATAACT GAAATTGGCC AAAAAGAATA 651 TCCTGATATT GACGTATCGT CCATCATTGT CGACAATGCC TCCATGCAGG 701 CGGTGGCCAA ACCTCATCAA TTTGATGTCC TAGTTACCCC TTCAATGTAC 751 GGTACCATCT TAGGCAACAT TGGCGCTGCT TTGATCGGTG GTCCAGGATT 801 GGTGGCAGGT GCCAACTTTG GCAGGGACTA TGCTGTCTTC GAACCAGGTT 851 CCAGACATGT TGGTTTAGAT ATTAAAGGCC AAAATGTGGC TAACCCAACT 901 GCCATGATCC TTTCCTCCAC GTTAATGTTG AACCATTTGG GTTTGAATGA 951 ATATGCTACT AGAATCTCAA AGGCAGTTCA TGAAACGATC GCAGAAGGTA 1001 AGCATACCAC TAGAGATATT GGTGGTTCCT CTTCTACTAC TGACTTCACG 1051 AATGAAATCA TCAACAAATT ATCTACCATG TAA
(SGD, 1997)
1 MLNRTIAKRT LATAAQAERT LPKKYGGRFT VTLIPGDGVG KEITDSVRTI 51 FEAENIPIDW ETINIKQTDH KEGVYEAVES LKRNKIGLKG LWHTPADQTG 101 HGSLNVALRK QLDIYANVAL FKSLKGVKTR IPDIDLIVIR ENTEGEFSGL 151 EHESVPGVVE SLKVMTRPKT ERIARFAFDF AKKYNRKSVT AVHKANIMKL 201 GDGLFRNIIT EIGQKEYPDI DVSSIIVDNA SMQAVAKPHQ FDVLVTPSMY 251 GTILGNIGAA LIGGPGLVAG ANFGRDYAVF EPGSRHVGLD IKGQNVANPT 301 AMILSSTLML NHLGLNEYAT RISKAVHETI AEGKHTTRDI GGSSSTTDFT 351 NEIINKLSTM (SGD, 1997)
Genome
Restriction Map for IDH1:
PCR primers for IDH1:
Forward: 5'
ATGCTTAACAGAACAATTGC 3' Tm = 46° C
Reverse: 5'
TTACATGGTAGATAATTTGTTG 3' Tm = 46° C
HIS HIS HIS HIS
HIS HIS MLNRTIAKRT
NAD+
Isocitrate Dehydrogenase Main Page
Created by: Elizabeth Shafer. Email
questions to lishafer@davidson.edu