*This page is part of
undergraduate assignment for Molecular Biology at Davidson College
Cloning 5 Isoforms
of Isocitrate Dehydrogenase
IDH2
Genebank
Accession Number: NC_001147
DNA Sequence
1 ATGTTGAGAA ATACTTTTTT TAGAAACACC TCGAGGAGGT TTTTGGCTAC 51 TGTAAAGCAA CCTTCAATCG GAAGATATAC CGGCAAACCT AACCCTTCTA 101 CCGGCAAATA CACGGTCTCG TTCATTGAAG GTGATGGTAT CGGACCTGAA 151 ATTTCCAAGT CTGTAAAGAA AATCTTTAGT GCAGCAAACG TCCCCATAGA 201 ATGGGAATCT TGTGATGTTA GCCCTATCTT TGTCAACGGA TTAACGACCA 251 TTCCTGACCC TGCCGTACAA TCTATCACAA AAAACCTGGT TGCACTAAAA 301 GGTCCACTAG CTACACCTAT TGGTAAAGGT CACAGATCTT TGAATTTGAC 351 ATTGAGAAAA ACATTTGGGT TATTTGCCAA CGTTCGTCCC GCAAAGTCTA 401 TTGAAGGTTT TAAGACCACT TACGAAAACG TTGATTTAGT TCTTATCAGA 451 GAGAATACCG AAGGTGAATA TTCTGGTATC GAACACATAG TTTGCCCTGG 501 CGTTGTTCAA TCTATTAAAC TGATCACAAG AGATGCCTCT GAGCGAGTCA 551 TTAGATACGC TTTTGAATAT GCAAGAGCCA TCGGCAGACC AAGAGTTATT 601 GTGGTACATA AATCTACTAT CCAGAGATTA GCTGATGGTT TATTCGTTAA 651 TGTTGCCAAA GAACTATCCA AAGAGTATCC TGACCTTACT TTAGAAACTG 701 AACTTATTGA TAACAGTGTG TTAAAGGTGG TCACCAACCC ATCTGCTTAC 751 ACAGACGCTG TTTCTGTTTG TCCAAATCTA TACGGTGATA TCTTGTCCGA 801 CTTGAACTCT GGTTTGAGCG CCGGTTCTTT AGGTTTAACT CCATCTGCCA 851 ATATTGGTCA TAAAATCTCG ATCTTTGAAG CTGTCCATGG CTCTGCCCCT 901 GATATTGCCG GTCAAGATAA AGCGAATCCA ACTGCCCTAC TTTTATCTTC 951 AGTAATGATG TTAAACCACA TGGGTCTAAC GAATCATGCT GACCAAATTC 1001 AAAATGCAGT ATTGTCTACT ATCGCATCAG GTCCAGAAAA CAGAACAGGT 1051 GACTTGGCTG GTACTGCTAC TACTTCATCA TTCACTGAAG CAGTCATCAA 1101 GAGATTATAA (SGD 1997)Amino Acid Sequence: 1 MLRNTFFRNT SRRFLATVKQ PSIGRYTGKP NPSTGKYTVS FIEGDGIGPE 51 ISKSVKKIFS AANVPIEWES CDVSPIFVNG LTTIPDPAVQ SITKNLVALK 101 GPLATPIGKG HRSLNLTLRK TFGLFANVRP AKSIEGFKTT YENVDLVLIR 151 ENTEGEYSGI EHIVCPGVVQ SIKLITRDAS ERVIRYAFEY ARAIGRPRVI 201 VVHKSTIQRL ADGLFVNVAK ELSKEYPDLT LETELIDNSV LKVVTNPSAY 251 TDAVSVCPNL YGDILSDLNS GLSAGSLGLT PSANIGHKIS IFEAVHGSAP 301 DIAGQDKANP TALLLSSVMM LNHMGLTNHA DQIQNAVLST IASGPENRTG 351 DLAGTATTSS FTEAVIKRL(SGD 1997)Gene Restriction Fragment Map of IDH2:
IDH2 PCR Primers:
Forward: 5'
ATGTTGAGAAATACTTTTTTTTAG 3' Tm = 45° C
Reverse: 5'
TTATAATCTCTTGATGACTG 3' Tm = 44° C
In Frame Fusion Protein:
HIS HIS HIS HIS HIS HIS MLRNTFFRNT
IDH2 Coenzyme:
NAD+
Isocitrate Dehydrogenase Main Page
Created
by: Elizabeth Shafer. Email questions to lishafer@davidson.edu