*This page is part of
undergraduate assignment for Molecular Biology at Davidson College
Cloning 5 Isoforms
of Isocitrate Dehydrogenase
IDP2
Genebank Accession Number: NC_001144
DNA Sequence
Amino Acid Sequence
Gene Sequence (with introns):
1 ATGACAAAGA TTAAGGTAGC TAACCCCATT GTGGAAATGG ACGGCGATGA 51 GCAAACAAGA ATAATCTGGC ATTTAATCAG GGACAAGTTA GTCTTGCCCT 101 ATCTTGACGT TGATTTGAAG TACTACGATC TTTCCGTGGA GTATCGTGAC 151 CAGACTAATG ATCAAGTAAC TGTGGATTCT GCCACCGCGA CTTTAAAGTA 201 TGGAGTAGCT GTCAAATGCG CGACTATTAC ACCCGATGAG GCAAGGGTCG 251 AGGAATTTCA TTTGAAAAAG ATGTGGAAAT CTCCAAATGG TACTATTAGA 301 AACATTTTGG GTGGTACAGT GTTCAGAGAA CCTATTATTA TCCCTAGAAT 351 TCCAAGGCTA GTTCCTCAAT GGGAGAAGCC CATCATCATT GGGAGACACG 401 CATTCGGCGA TCAGTACAAA GCTACCGATG TAATAGTCCC TGAAGAAGGC 451 GAGTTGAGGC TTGTTTATAA ATCCAAGAGC GGAACTCATG ATGTAGATCT 501 GAAGGTATTT GACTACCCAG AACATGGTGG GGTTGCCATG ATGATGTACA 551 ACACTACAGA TTCGATCGAA GGGTTTGCGA AGGCCTCCTT TGAATTGGCC 601 ATTGAAAGGA AGTTACCATT ATATTCCACT ACTAAGAATA CTATTTTGAA 651 GAAGTATGAT GGTAAATTCA AAGATGTTTT CGAAGCCATG TATGCTAGAA 701 GTTATAAAGA GAAGTTTGAA TCCCTTGGCA TCTGGTACGA GCACCGTTTA 751 ATTGATGATA TGGTGGCCCA AATGTTGAAA TCTAAAGGTG GATACATAAT 801 TGCCATGAAA AATTACGACG GTGACGTAGA ATCAGATATT GTTGCACAAG 851 GATTTGGCTC CTTGGGGTTA ATGACATCTG TGTTGATTAC CCCGGACGGT 901 AAAACCTTTG AAAGCGAAGC CGCCCACGGT ACAGTAACAA GACATTTTAG 951 ACAGCATCAG CAAGGAAAGG AGACGTCAAC AAATTCCATT GCATCAATTT 1001 TCGCGTGGAC TAGAGGTATT ATTCAAAGGG GTAAACTTGA TAATACTCCA 1051 GATGTAGTTA AGTTCGGCCA AATATTGGAA AGCGCTACGG TAAATACAGT 1101 GCAAGAAGAT GGAATCATGA CTAAAGATTT GGCGCTCATT CTCGGTAAGT 1151 CTGAAAGATC CGCTTATGTC ACTACCGAGG AGTTCATTGA CGCGGTGGAA 1201 TCTAGATTGA AAAAAGAGTT CGAGGCAGCT GCATTGTAA
(SGD
1997)
1 MTKIKVANPI VEMDGDEQTR IIWHLIRDKL VLPYLDVDLK YYDLSVEYRD 51 QTNDQVTVDS ATATLKYGVA VKCATITPDE ARVEEFHLKK MWKSPNGTIR 101 NILGGTVFRE PIIIPRIPRL VPQWEKPIII GRHAFGDQYK ATDVIVPEEG 151 ELRLVYKSKS GTHDVDLKVF DYPEHGGVAM MMYNTTDSIE GFAKASFELA 201 IERKLPLYST TKNTILKKYD GKFKDVFEAM YARSYKEKFE SLGIWYEHRL 251 IDDMVAQMLK SKGGYIIAMK NYDGDVESDI VAQGFGSLGL MTSVLITPDG 301 KTFESEAAHG TVTRHFRQHQ QGKETSTNSI ASIFAWTRGI IQRGKLDNTP 351 DVVKFGQILE SATVNTVQED GIMTKDLALI LGKSERSAYV TTEEFIDAVE 401 SRLKKEFEAA AL(SGD 1997)
Gene
Restriction Fragment Map:
IDP2 PCR Primers:
Forward: 5'
ATGACAAAGATTAAGGTAGC 3' Tm = 46° C
Reverse: 5'
TTACAATGCAGCTGCCTCGA 3' Tm = 52° C
HIS HIS HIS HIS HIS HIS MTKIKVANPI
NADP+
Isocitrate Dehydrogenase Main Page
Created
by: Elizabeth Shafer. Email questions to lishafer@davidson.edu