Affinity chromatography is designed to purify a particular protein from a mixed sample.
Figure 1. Loading affinity column.
Figure 2. Proteins sieve through matrix of affinity beads.
Figure 3. Proteins interact with affinity ligand with some binding loosely and others tightly.
Figure 4. Wash off proteins that do not bind.
Figure 5. Wash off proteins that bind loosely.
Figure 6. Elute proteins that bind tightly to ligand and collect purified protein of interest.
© Copyright 2002 Department of Biology, Davidson College, Davidson, NC 28036
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