Gene Networks Database

Strongylocentrotus purpuratus Genes in Development: Primary mesenchyme-specific genes

PM27

Function

PM27 gene codes for the 27 kDa primary mesenchyme-specific spicule protein.
PM27 plays a role in the regulation or execution of skeletal growth, as opposed to maintenance or structural integrity of the spicules (Harkey M.A. et al,1995).

Protein

The PM27 sequence predicts a nonglycosylated secretory product of 27 kDa in mature form.
The N-terminal "repeat" domain of the amino acid sequence consists of a series of tandem repeats and shares both sequence and predicted structural similarities with several fiber-forming proteins.
The C-terminal "lectin" domain is similar to the C-class lectins.
Antisera against either domain of PM27 detect two major proteins in embryo extracts, with apparent molecular weights of 27 and 30 kDa (Harkey M.A. et al,1995).
SWISS_PROT: Q26616

Subcellular location

Expression Pattern

Both the transcript and encoded protein appear at the mesenchyme blastula stage and are restricted to the primary mesenchyme cell (PMC) lineage throughout development. Experiments showed that this cell specificity is regulated at the level of transcription.
PM27 is produced uniformly and exclusively by PMCs through the early prism stage; this specificity is further restricted during skeletogenesis to a subpopulation of PMCs associated with the growing tips of the spicules. PM27 is secreted to the skeletal compartment and accumulates predominantly at the advancing mineralizing surface of the spicule tips.
As the spicules elongate PM27 protein disappears from the more mature mid-shaft regions (Harkey M.A. et al,1995).
During skeletogenes PM27 is expressed coordinately with another structural gene, msp130 (Raman V. et al,1993).

mRNA level

Temporal accumulation

Method: Nothern blot hybridization
Reference: Harkey et al., 1995

Stage16-cell (7 hr)
Hatching blastula (20 hr)
 Early mesenchyme blastula
(25 hr)
Early gastrula (35 hr)
 Early prism
(50 hr)
Early pluteus (70 hr)
Late pluteus (97 hr)
Level (molecules per cell)
-
1,2
5
15,5
17
17
9,2

Spatial localization

Method: Whole mount immunolocalization
Reference: Harkey et al., 1995

Stage
Egg
16-cell
Mesenchyme blastula
Gastrula
Prism
Pluteus
Tissue
-
-
 PMCs PMCs, mainly PMCs of the triradiate spicule rudiments
PMCs, mainly PMCs near the growing tips
 PMCs, mainly PMCs near the tips of the body rod

Sequences

GenBank:

Regulatory Regions

Regulatory Connections

Upstream Genes

PM27

Downstream Genes

Evolutionary homologues

Links

Bibliography
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Comments are welcome to Sveta Surkova
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