Gene Networks Database

LOCUS       5FR_SPSM30     2963 bp    DNA             INV       18-NOV-1994
DEFINITION  Strongylocentrotus purpuratus spicule matrix SM30-alpha protein
            (SM30) gene, 5'-flanking region
DBSOURCE    GENBANK:  name SPSM30S1, accession U05961 
KEYWORDS    
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Akasaka,K., Frudakis,T.N., Killian,C.E., George,N.C., Yamasu,K.,
            Khaner,O. and Wilt,F.H.
  TITLE     Genomic organization of a gene encoding the spicule matrix protein
            SM30 in the sea urchin Strongylocentrotus purpuratus
  JOURNAL   J. Biol. Chem. 269 (32), 20592-20598 (1994)
  MEDLINE   94327638
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Killian,C.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-1994) Christopher E. Killian, University of
            California, Berkeley, Molecular and Cell Biology, Life Science
            Addition, Room 379, Berkeley, CA 94720, USA
REFERENCE   3 
AUTHOR      Frudakis,T.N., Wilt,F.H.  
TITLE       Two cis elements collaborate to spatially repress
            transcription from a sea urchin promoter
 JOURNAL    Dev Biol. 172(1): 230-241 (1995)
  MEDLINE   96069917
COMMENTS    Chloramphenycol acetyl transferase assays of deletion constructs
            from the 2600 upstream region showed that the region from -1628 
            to -300 contains repressive elements that are responsible for 
            a mesenchyme-specific pattern of SM30 gene transcription. Two 
            highly repetitive sequence motifs, (G/A/C)CCCCT and (T/C)(T/A/
            C)CTTTT(T/A/C) are present in this region. Representatives
            of these elements were analysed by gel mobility shift experiments
            and were found to interact specifically with proteins in crude
            nuclear extracts. Only composite element containing both types
            of elements is sufficient to function as a tissue-specific repressive
            element [3].
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /clone_lib="lambda gemm 11 genomic library made by N.
                     George"
                     /tissue_type="sperm"
                     /clone_lib="lambda gem11 genomic library made by N.
                     George"
     control_region  1011..2339     
                     /citation=[3]
     TATA_signal     2609..2615
     mRNA            2639..
                     /evidence=experimental
ORIGIN      
        1 gtcgacatag tcgtatcata attttaattg taatgatgac gatgattatg gtaatgatgt
       61 tgatggcgat aatgatgacg atgatgatga tgatgatgat gatgattatg atggtgatga
      121 tgatgatgat gatgataatg atgaggatga taatgataac aacgatattg atgaaaatgc
      181 tagtaatact taaaatattt ataacaattt caacgacaat aatacgacc aaaaaaagaa
      241 aatgcactac aggagatgaa tatattgagg aacataataa tatttataat aacgatgtag
      301 ggactatgat cattattgaa gtgattaatc atgtatatat catgtcgcgc aaggtaacaa
      361 gggatgactt ccaaagtttt tgtaaaagtc acgatagaga acgaaaagta tcaaaccagc
      421 aatttcaact tacacacatt acaacactca aaagagctat aaaaattgat attttaaaac
      481 aaagaaatga aattaacaat gtcagtatct gcaaagtcaa tgtcaaatat aactctgaat
      541 gtaaactaac tgtcatgtaa tctttagaag atacttatta ttcatttatt taaagtggat
      601 aaagttaaca agaatgctta ttataatcta ggaagtcgat atttcttgag tagtgtagcg
      661 caaatagttt gtgagagttt cggtggaaaa atgtggtaaa cagtgtgtta ttcttaaact
      721 acacattgca cacaaagatt ggttatgtca tcaccagcgc tattctaaca ctagacggtt
      781 tctgataggt gatagccagt gcgccaataa tcaaacgatg tttgcacagg gtatttggta
      841 ataaggcaaa atttaaaaaa ataaaacgtg gtttatttct aattcgatac cagtgattta
      901 caaatgttgc tgaaatgaca tgattttccg ttttttctct aatttttgcc cattatgcca
      961 gaggcggcac taccggtggg gtggtggcac tgtgccaccc acttttccag gtacccacaa
     1021 acaacccatt tttacatcga acaagtgccc cttttctttt ataataactg tccttttcct
     1081 ctcttttcat ctctggtgag taggcctttt cggtgcgaga aattcttttt ttcacctctg
     1141 atattttttg cccttttttt tattttactt tgactcgtgc cccttttact ttttaataag
     1201 tatacttttt ccttttaata agaaccccct tcactttctc tgaaatgcca cttttcacct
     1261 ctgataagtg ctccttttcc ttttttatat gtgcccctcc cccttttcaa aaaccccttt
     1321 taacatctga taaatgcccc tatcaaattc tgataggtgc ccttctcctc caacagattc
     1381 cacacttcca ttctcacaag tgcctcattt cattactgtt gagtgtccct tttcaggagc
     1441 aagaagttcc atcacctctt aaggatctga taattgccct tttactttga cacgaacacc
     1501 ttttcttttc tgaaaactgt cttttctttt ttgataagtg cctacttttc ccttctccat
     1561 aacatgcact ccctaatcca aggcgattta gagctccttt ttctttcatt ttttcctaga
     1621 gcttgctcgc tacgctcgct cgcatttata gatgttaaat tatgttacta atatgaaaag
     1681 aacttctcag tctttcttta ttagattggg cttaatactc atcagggatt tggttttctt
     1741 aataagatat aatttttggc aatggacctc acaaatctta atgtttttgc tcgcacgctt
     1801 cgctagttcg aacattaata ttcagaccat caccgttaca cctaactgca aagtatgttg
     1861 cttaagtaag cagtatcttg taccccttaa agaaagaaac caatattgat atcatccctg
     1921 gctgtgtaat cacaatcatg acgatgtagg ggtatatacc aatgtcttaa aatggtgtag
     1981 gttttcttca tgtttccctt tgtaagtgcc ccctttttcg agaaagtgcc ctttttggtt
     2041 ggtgccccct acctactttg gaagacgctc cgccgcccct gcattatgct acttggtggt
     2101 gataaggatt ttgttttgac ccctcaaaag ttgttggtca ttacgccact atctgtacgg
     2161 ggttagtgtc ctgctgcgat gtggttaact cttagtatca tgaggtatat agcaagcaat
     2221 cgtgtacagc aatggcttag ctagattgag ataggggaca aataccccct ccctaattga
     2281 atgacgaaac ataaaaagga agaagataaa ggttgttatc attttgtcaa tcattcgaat
     2341 tgctaaataa agattcaaga tctgtgacaa ataaatgagg attagtcatc cattgttacc
     2401 taacctcact gtctcctgca ccagcgcctc tctctcattc acacttcaat atcgtcatca
     2461 tccatcgccc ctcctacttt ttgtttgacc acggacatta cagtggtgac cggaagctaa
     2521 cgtaataagt gtgcgttgtc aactctgcat cgcgcttcga gatgggggat actgattgac
     2581 aaaagtctgt ccgatccaaa atgtatcgta taaataggac tgtcacacct ggccaaatag
     2641 gataatacca tcgtcatcga ctcgagttgg tcagctcaga atccaccttt aggtctctcc
     2701 tccaagcttc agttaaacca ctccacggaa tctttcttca tctagaagtc tcgcacgttg
     2761 tccaagaaga tgaggggttt cgtctatgtg ttggtgtgcg tattggcttt ggcctctttc
     2821 tcccgagccc agttgcccgg tggtggcggt cccgttttac cgggtggagg accaaccata
     2881 ggaccagtga atccagaccc gaccaggaca gaagtatgcg ccaagttctg ggtacaagaa
     2941 ggaaacagct gctacctgta agt
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