sterile yellow and blue tips
sterile culture tubes
sterile tooth picks
LB + amp. agar plates	make 1 L
5M NaCl	make 100 mL
1 M Tris (pH 8.0)	make 1 L
0.5 M EDTA	make 500 mL
5 M NaOH	make 100 mL
10X tris saline	make 125 mL
10X transfer buffer (western)	make 500 mL
20X SSC	make 1 L
ECL 2° Wash	make 2 L
Ampicillin stock	ask Dr. C.
5X TBE	make 1 L

Reagent	Volume	Container	Autoclave?
sterile yellow tips	1 box per person	yellow box	YES
sterile culture tubes	1 full blue rack	blue rack	YES
sterile tooth picks	1 beaker per lab day	small beaker + foil	YES
LB+amp. agar plates	1 L	2 L Flask	YES + amp and pour
0.5 M EDTA	500 mL	1 L bottle	NO
5M NaCl	100 mL	100 mL bottle	NO
5 M NaOH	100 mL	100 mL bottle	NO
10X tris saline	125 mL	250 mL bottle	NO
Turbo Denature Buff.	2 L	2 x 1 L bottles	NO
10X transfer stock (western)	500 mL	500 mL bottle	NO
20X SSC	1L	1L bottle	NO
ECL 2° Wash	2 L	2 x 1L bottle	NO
Ampicillin Stock	ask Dr. C.	blue cap 15 mL tube	filter sterilize
5X TBE	1 L	1 L bottle	NO

sterile yellow tips
sterile culture tubes
sterile tooth pick
LB liq. medium	make 500 mL
LB + amp agar plates	make 1L
3 M sodium acetate	make 50 ml you figure this one out (pH to 5.2 with glacial acetic acid)
Turbo Transfer Buffer	make 2 L
Turbo Denature Buffer	make 2 L
Turbo 5X Neutralizing Buffer	make 500 mL
ECL primary wash (w/o urea)	make 2 L
ponsceau	make 100 mL
1X stock of TE	50 mL
5X TBE	make 1 L

 

Reagent	Volume	Container	Autoclave?
sterile yellow tips	1 box per person	yellow box	YES
sterile culture tubes	1 full blue rack	blue rack	YES
sterile tooth picks	1 beaker	small beaker + foil	YES
LB media, no amp	500 ml	five 100 ml bottles	YES
LB+amp. agar plates	1L	in a two liter flask	YES + amp and pour
3 M sodium acetate	50 ml	100 ml bottle	NO pH = 5.2: glacial acetic
Turbo Denature Buff.	2 L	2 x 1 L bottles	NO
Turbo Trans. Buf.	2 L	2 X 1 L bottle	NO
Turbo Neut. Buf.	200 ml	500 ml bottle	NO
ECL 1° wash	2 L	2 X 1 L bottle	NO
1X stock of TE	50 mL	100 mL bottle	YES
ponsceau	100 ml	100 ml bottle	NO
5X TBE	1 L	1 L bottle	YES

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Lab Safety