Proteomics by 2D Gels
			
		
		
			
Two-dimensional Gel Electrophoresis
		
		
			
			
		
		
			
Cut out Spots and Identify Proteins by Tandom Mass Spectroscopy
		
		
			
			
		
		
			
Each peptide could be broken into many possible smaller fragments
		
		
			
			
			
		
		
			
Each ion will have its own mass to charge ratio
		
		
			
			
		
		
			
The real spectrum is compared to all possible spectra for a given species and the peptide sequence is identified
		
		
			
			
			
		
		
			
  (animation 
    and images courtesy Stan Fields)
  Overview Page
		
		
		
		
			Genomics Course Page
			Biology Department Main Page
			
 
		
		
		
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