EVOLUTION Fall 2004: Review Key 3

Spend no more than 3.5 hours on this review.  Please type your answers on this review and email the whole thing back to me by 11:59 pm Thursday night.  Do not put your name ANYWHERE on the review other than at the bottom of the last page (your pledge).  Pace yourself.  I’ve enjoyed you guys and the experience of making myself think about topics with which I was unfamiliar.

 

THIS ANSWER KEY IS THE REVIEW OF THE STUDENT WHO MADE THE HIGHEST GRADE

 

1. Describe where my office is located. (1 pt extra credit)

 

2. What is a reaction norm and what relationship, if any, does it have to canalization? (4 pts)  A reaction norm is the possible phenotypes that could emerge from a single genotype when it is exposed to a range of environmental conditions.  Canalization can be considered to be the force which could limit the range of possible reaction norms.  Canalization will be selected for when there is stabilizing selection on a given allele (or multi-allelic trait) under a wide range of environmental conditions.  In other words, there is a desirable phenotype (+/- a small amount of acceptable deviation from this optimum) that exists under a wide variety of environmental conditions.  For example, there is a great deal of stabilizing selection for neurons to function the way that they do-even if you are born at high altitudes your neurons will be similar, if not functionally identical, to low-altitude neurons.  Thus, neuron-function could be considered to be a highly canalized trait, and the reaction norm for this trait is limited.   

 

3. What is the benefit of developmental plasticity? (3 pts)  Developmental plasticity allows for an organism to produce a phenotype that is most closely matched to its environment.  This would allow for close matching of phenotype to a given environment. 

 

4. What is meant by a cost of developmental plasticity? (2 pts)  A cost of developmental plasticity is a cost, in terms of fitness (can subdivide fitness into its components-survival, reproduction) which an organism pays in order to either be plastic or use its plasticity. 

 

Provide 2 examples. (4 pts)  An example of the cost of being plastic would involve when a developmentally fixed organism can produce a given trait more efficiently than can a plastic organism.  For instance, if two different races of cladoceran produce a spike, and one always does and the other does in response to predator presence, in an environment with predators, if producing the spike plastically is more expensive, then the plastic organism would pay a cost for being plastic.  Another example of the cost of plasticity involves when using a plastic trait the trait has negative effects, by means of epistatic interactions, on a phenotype that is either completely unassociated or only marginally associated with the plastic trait . 

 

5. What is meant by a limit of developmental plasticity? (2 pts)  I think that both costs and limits of plasticity can only be determined with respect to a fixed trait, and that limits of plasticity are necessarily relative.  A limit of developmental plasticity is when the range of plastic phenotypes that can be produced is somehow limited with respect to the range that can be achieved through fixed development, or when the phenotype that can be produced plastically cannot achieve the optimum phenotype as well as a developmentally fixed organism. 

 

Provide 2 examples. (4 pts)  A limit of developmental plasticity would be when in an extreme environment a plastic organism cannot produce the phenotype that is optimum as well as a developmentally fixed organism.  Another limit of developmental plasticity is due to information.  An organism must rely on information, and then process it, and then incorporate this information as it develops the plastic trait.  If information is limited, or if an organism is correct initially but the environment changes, then the organism will be unable to produce a phenotype that is correctly matched to the environment. 

 

6. “A given environment-dependent phenotypic change will be called plasticity if it is believed to be adaptive, and lack of canalization if it is not.”  Explain. (4 pts)   Both canalization and plasticity implicitly imply that the phenomena are adaptive.  Plasticity allows for a certain range of phenotypes to be expressed in accordance with the environment that the organism is exposed to.  Thus it is assumed to be adaptive for an organism to allow for a “wide”-ranged reaction norm, with reference to a specific trait or traits.  When referring to another trait, one which the investigator believes to be highly conserved across environmental gradients due to strong stabilizing selection, variation or deviance from the “optimum” is assumed to be non-adaptive, and thus could be characterized as a lack of canalization.  When a trait is considered to be highly canalized, it is assumed that variation from the norm is fitness-reducing, and canalization is the process by which a trait is produced to be as close as possible to an optimum. 

 

7. Why might some people argue that characters for which asymmetry largely affects fitness might exhibit less fluctuating asymmetry than other traits. (4 pts)  Well, for one thing, ideally symmetrical characters, such as wing structure and shape, will deteriorate in function as they become less symmetrical.  So from a purely functional standpoint (and consequently fitness-a lop-sided bird isn’t able to control flight and catch as many insects and won’t have as much time to sing, attract mates, and protect a territory), symmetry leads to more precise function.  With respect to characters such as sexual traits, in which symmetry is selected for as because it is indicative of overall quality, symmetry is a signal of developmental coordination and efficiency.  If a male cardinal is symmetrical, one could speculate that he has good genes which allow him to be symmetrical.  Females will choose him because his symmetry is indicative of his overall quality.  So don’t stray from the symmetry. 

 

8. Like predators, parasitoids must kill their hosts.  So how is it that they can be said to vary in virulence? (4 pts)  It can be said that they vary in virulence by describing the proportion of the hosts which they effectively colonize and kill.  A 100% virulent parasitoid would never be encapsulated and destroyed by the host insect—it would always effectively use the host’s resources to grow and eventually destroy the host as it developed.  A certain parasitoid will vary in its virulence with respect to different hosts, and a single host race will vary in the virulence that different parasitoids demonstrate.  Also affecting parasitoid virulence are environmental conditions, such as host condition and the temperature to which the host/parasitoid system is exposed.    Here, variation in temperature can either increase or decrease parasitoid virulence. 

 

9. How might the sex ratio produced by a female insect infected by Wolbachia be affected by a short-term exposure to high temperatures? Briefly explain. (3 pts)  A female insect which was exposed for a short time to high temperatures would be more likely to produce an even sex ratio.  Wolbachia influences female insects to only lay female eggs, because, like mitochondria, endosymbionts are only passed on in the female germ line.  Thus, males do not contribute to the fitness of the Wolbachia strain.  When a female is exposed to high temperatures, she might clear herself of her Wolbachia load, as the endosymbiont has a lower LTmax than most insects (perhaps not a Grylloblatid, a crazy little insect that lives in glacier fields and far above treeline). 

 

10. Briefly explain the three characteristics of H. floresensis that make this find ”among the most outstanding discoveries in palaeoanthropology for half a century”? (6 pts)  It’s very small size- the Flores man was apparently little more than a meter tall, and had a brain case much smaller than modern humans.   It’s apparent persistence until almost 18,000 (?) years ago, only 8,000 years before H. sapiens showed up in Indonesia.   And it’s apparent use of tools and weapons, suggesting a high degree of technological sophistication-on the site were found what were believed to be rudimentary weapons and the bones of komodo dragons and extinct pygmy elephants, suggesting that H. floriensis was a social and coordinated hunter (which may mean that it even had language, but this will most likely never be known).    

 

11. One of these characteristics is the reason why the remains on Flores have been called “the most extreme example ever found of human adaptation.”  That’s a very bold statement.  Explain. (3 pts)  This statement is referring to the small size of the hominids found on Flores.  Along with pygmy elephants and massive lizards, they are an example of the strange forces that islands can work on the evolution of organisms.  Due to some sort of constraint, be it resources or something else, H. floriensis became much smaller than its likely ancestor H. erectus.  So human-like creatures can be as flexible as the next type of critter, getting smaller due to environmental constraints.   

 

12. What is the African Replacement Model of human evolution? (4 pts)  The African replacement model is that H. sapiens evolved in Africa and migrated to replace the rest of the hominid species that had arisen and migrated around the earth.  This model assumes that there was little or no interbreeding/hybridization between the H. sapiens and neandertalensis or erectus.  Homo sapiens came to eventually swamp the rest of the hominid species. 

 

13. In our discussions, we mentioned fever as a non-specific response.  That was an over-statement.  Explain what’s going on in the following (real) scenarios:

 

Species A exhibits a high fever when attacked by pathogen X and recovers. (2 pts)  Species A is either a) attempting to destroy directly the pathogen by means of heating it beyond its LTmax, or b) attempt to affect its own immune system in a way that will allow it to overcome the pathogen.  Either a) or b) or an interaction between the two processes is successful and Species A is able to recover. 

 

Species A exhibits a mild fever when attacked by pathogen Y and recovers. (4 pts)  I’m not sure that I understand what the difference would be between a mild fever and severe fever, other than the intensity of the infection which must be overcome.  When there is a serious infection, such as the flu or Streptococcus pneumoniae, the immune response will be greater than when there is an infection of the common cold, simply because there is a greater risk in allowing the flu pathogen to persist in the body.    So apparently, pathogen Y is not as severe of an infection, and that the interactive attack of the immune system on the pathogen does not require as significant of a fever response in order to be effective.  A fever condition is a risky condition to be in, as it will limit an organism’s ability to forage and otherwise fend for itself.  So if the pathogen is not as great of a potential risk, an optimum fever response would be milder than in the case of attack by a highly virulent pathogen. 

 

Species A exhibits a mild fever when attacked by pathogen Z and does not recover. (4 pts)  This seems the trickiest to me.  Apparently, for some reason, Species’ A immune response could be considered ill-matched to the nature of the pathogen and the risk that it posed.  Perhaps, although this seems unlikely, mild fever benefited the pathogen and increased its replication rate.  Or A’s immune system believed that the highly virulent and risk-posing pathogen was more like a little cold pathogen, and did not respond to the threat strongly enough.  This response by A would have to be deliberately induced by pathogen Z in order for this low fever/death sequence to be evolutionary beneficial for Z.  If it was not adaptive, this would represent a dead end for species Z.  This seems likely because low fever would usually indicate low pathogen virulence and hence low rate of pathogen replication and consequently a low rate of pathogen transmission.  If a pathogen has a low rate of pathogen transmission, it would be adaptive to keep the host alive so that the host can interact with a large number of potential hosts.  (As I was answering question 17, I realized that species A could also be ectothermic, and is unable to raise its temperature sufficiently to overcome the pathogen because of environmental constraints). 

 

14. If a parasite/pathogen is locally adapted, infectivity rates will be higher in sympatric hosts vs. allopatric hosts.  Does the same relationship hold for virulence?  Explain your answer. (4 pts)

Not necessarily.  Optimum virulence is adaptive in the sense that infectivity is maximized.  Infectivity can be maximized by a variety of different optimum virulence strategies.  Each particular optimum virulence strategy is a function of local host ecology.  The conditions of host habitat influence pathogen virulence.  Pathogen virulence will often be greater in poor-condition habitats, due to high host mortality.  The behavioral ecology (social, eusocial, not social- interaction frequency) of the host will also influence parasite virulence.  In highly social or gregarious animals, high virulence could be selected for.  Related to this is local variation in pathogen dispersal mechanism.  If pathogen dispersal mechanisms vary spatially, optimum virulence will vary according to the dispersal mechanism. 

 

15. In the data paper about the wasps and Drosophila, if several generations of selection changed encapsulation rates from 10% to 60% and if encapsulation prevents mortality, why were encapsulation rates only 10% in the first place? (and please define encapsulation). (3 pts)  Encapsulation is a surrounding of parasitoid egg in insects by haemocyte attack.  Haemocytes attack the egg, and either destroy it by direct attack or asphyxiate it.   There will be selection for the ability to encapsulate the egg.  Host defenses in the first generation were ill-prepared for the threat that the parasitoid, at least most of them.  Those flies who had some sort of beneficial variation from the majority in immune response were able to encapsulate the egg (this incidence seems like good empirical evidence of the Red Queen hypothesis).  These flies lived to reproduce and a larger proportion than 10% of their offspring inherited the allelic combination which proved to be effective against the parasitoid.  This process repeated until encapsulation rates achieved 60% (the same process will occur, but in reverse, as parasitoid eggs are selected for their ability to overcome host defenses). 

 

16. You’re a biocontrol expert working for the UN in southern Africa.  In spring you test a new fungus for its ability to infect and kill locusts.  It works fabulously, so you move to implement it as a biocontrol agent as quickly as possible.  By summer you’ve jumped through all sorts of bureaucratic and industrial hoops and you start infecting plagues of locusts.  But it doesn’t work.  Why not? (3 pts)  It must be related to the seasonal changes which the question makes explicit.  Spring is cooler than summer and the angle at which the sun strikes the earth makes thermoregulation less effective, so the locust is unable to raise its internal temperature sufficiently to overcome the pathogen.  But by late summer, thermoregulatory opportunities are greater and ambient temperatures are much higher, so that the locust is able to raise its temperature sufficiently to overcome the parasite.  Parasites on ectothermic animals often have temperature preference and tolerance curves that are shifted towards the cold side, respective of the host organism. 

 

17. Not all reductions in host fitness are due to pathogen multiplication.  How does this pose a problem for the trade-off hypothesis? (4 pts)  The trade-off hypothesis assumes that reductions in host fitness and survival are the product of a trade-off between parasite replication and parasite transmission, and that there is selection for the parasite to achieve some sort of optimum virulence, suited to its particular mode of transmission.  Thus, host fitness in this hypothesis is decreased in relation to the virulence (pathogen multiplication) exhibited by a particular parasite.  The tradeoff hypothesis could fail to explain observed phenomena when evolutionary forces fail to act upon a particular strain or colony of pathogen.  For instance, polio normally affects the gastro-intestinal trait, and has oral/fecal route of transmission.  But when polio enters the CNS, there is no selection for optimal levels of virulence, and the virus replicates beyond bounds suitable for optimum transmission. 

 

18. This first paper we read this time said “One of the most important advances achieved through the evolutionary synthesis is the consideration of phenotypic variation as a quantity of interest, whereas, according to the classic typological view, it is considered a nuisance.”  Nonetheless, most biologists are still guilty of some sort of tunnel vision.  Explain why one biologist might legitimately study host-parasite relationships by ignoring variation among pathogens and instead injecting her subjects with sheep red blood cells… and explain why another biologist assumes all her subjects are equivalent and is interested only in the variation produced by injecting them with an allopatric pathogen vs. a sympatric one. (4 pts)  The first scientist is testing for strength of host response while controlling for pathogen- specific interactions with the host.  By injecting sheep red blood cells, she can take measurements such as antibody production or T-cell count.  She is attempting to quantify or describe induced non-specific response to a pathogen.  The second scientist is attempting to quantify variation in induced specific response to a pathogen by eliminating the variability in host-condition.  She is attempting to isolate the effects that parasite/host local coevolution have on the nature of disease.  Controlling for variable is a necessary measure to obtain information.  By stripping down or assuming away levels of complexity we are more easily able to generalize, draw conclusions, and test those conclusions at different levels of scale and complexity (see Simon Levin in Ecology McArthur Lecture from about 10 years back). 

 

19. “Virulence is not necessarily a constant, nor simply an inherent property of the parasite alone”

Explain how each of the following are expected to affect virulence:

Delay between transmission and mortality (4 pts)

As the delay between the time-period in which transmission is possible and mortality increases, virulence is expected to increase.  The author of the paper in which this idea was discussed made the analogy of disease to organismal life history and the evolution of senescence.  The same is true in organisms, when the mortality costs of a given reproductive effort are delayed, the more that a high level of reproductive effort will be selected for.  The same in the parasite; when mortality costs of a given level of virulence (and hence rate of transmission and transmission potential) are felt way down the line, possibly nearing the end of the time-frame in which transmission is possible, there will be selection for high levels of virulence.

Mortality rate of uninfected individuals (3 pts) 

When there is a high level of background mortality in the host population, there will be selection for a high level of virulence (if I’m not mistaken, I don’t think this point was cut and dry clarified in the paper, but I’ll do my best with logic).  If wombats are dropping left and right wombat parasites will be selected for a high level of virulence, because rapid exploitation (high virulence) of host resources will lead to greater transmission than low levels of host exploitation.  If you are a highly virulent strain, there is a greater likelihood that you will transmit to a new host before your host dies, which he is likely to do at any second, infection or no infection.

 

20. Like HIV, malaria has a high mutation rate that allows the plasmodium to evade our efforts at vaccines.  We said that condoms would not only slow the spread of HIV, but also select for less virulent forms.  Why would providing mosquito-netting for malaria patients (rather than for healthy persons) accomplish the same thing? (4 pts)  There is an evolutionary cycle that occurs in which those strains that are capable of rapid transmission are selected for high virulence, and those strains which are incapable of rapid transmission (due to the behavioral/spatial ecology of the host), are selected for low virulence.  This is so because high virulent strains decrease the timeframe of opportunity for transmission in “hopes” of accelerating the rate of transmission; low-virulence strains will evolve when the behavioral ecology or spatial structuring of the host prevents rapid transmission.  By preventing patients that have malaria from spreading the disease (via Anopheline mosquitoes), you are effectively slowing the rate of transmission, and thus selecting for low-virulence strains that will persist within the host for longer, and allow him/her to be able to walk around and get bitten by a skeeter. 

 

21. So… you are HIV positive and an intravenous drug user (a junkie). 

Why would you not want to share a needle with another junkie? (3 pts)  He could have a different strain of HIV, and by sharing needles you could develop a “super-infection,” or an infection consisting of multiple strains.  When this occurs, the strains will compete with each other to obtain resources from the host.   When this happens, “evolutionary stable” levels of virulence will be thrown out the window, and the virulence of the infection will dramatically increase, and the user/HIV + will be made extremely sick.

 

Why would you especially not want to share a needle with another junkie who’s AIDS has progressed to an advanced state? (3 pts)  First of all, by saying that he has AIDS means that his HIV has progressed to a degree where his T-cell count is very low, and thus he is very likely to have other infections besides AIDS (pneumonia, flu), which could have severe effects on you in your weakened HIV+ state.  Also, selection is not as strong on “old” infections to maintain an optimal level of virulence.  Thus, you could potentially contract one of these “pie-eating” strains of HIV, which would lead to rapid deterioration of your health. 

 

22. Explain Red Queen dynamics, what they have to do with host specificity, why they are invoked to explain the maintenance of sexual reproduction, and why they are invoked to explain the maintenance of heritable variation of fitness related traits. (8 pts)  So the Red Queen says to Alice, “You’ve got to keep running all the time just to stay in the same place.”  I’m paraphrasing from Lewis Carroll, who is the source of the name for the biological phenomenon.  Describes the dynamic, at times cyclical, process of coevolution of parasites and hosts.  Rare host genotypes that are capable of overcoming parasite attack are selected for and become more common—rare parasite genotypes that are able to overcome the now common host genotype’s defenses achieve high fitness and drive the common host genotype to extinction or very close to it.  This process continues ad infitum, which invokes the running in place but never getting anywhere image. 

Host specificity- Red Queen dynamics will tend to create extensive host/parasite coevolution.  The parasite will become very specialized at overcoming host defense mechanisms, so specialized that its approach would not be effective against different types of hosts.  Since immune response is such a highly coordinated and sophisticated system, a parasite can’t be some blundering yokel sort of parasite, walking in thinking it’s gonna take over the joint.  It would get shot down in a second by the coordinated host immune system.  Thus, parasites will tend to become increasingly specialized to over come a particular host’s defenses. 

Sexual reproduction has the potential to generate genetic diversity in one generation.  Asexual reproduction is only capable of generating genetic diversity by means of mutation and with high levels of mutation subsequently follow high levels of deleterious mutation.  Sexual reproduction generates the rare host genotypes, relatively free of deleterious mutations, which will be the next generation of rare defense-capable host organisms. 

Heritable variation of fitness related traits- As I stated above, there are rare host genotypes who are able to overcome parasite attack.  These genotypes will achieve high fitness and have lots of offspring.   These offspring, at least until parasite attack mechanisms catch up, will have high fitness.  

 

23. Explain Sewell Wright’s adaptive landscape model (4 pts)  Sewell Wright’s adaptive landscape model describes a series of fitness peaks, a sort of topographical map of adaptiveness.  I am not quite sure of the limitations of the model, and how one would actually quantify the position of a particular (extant) species on the landscape (it seems possible that one could outline historical positions, and demonstrate how an organism showed at Time A that it had high fitness, and was moving upwards, but an environmental disturbance at Time B knocked it from its adaptive peak).   Nevertheless, it is a useful model as it puts into a visual/graphical perspective the consequences of certain genetic, evolutionary, and environmental mechanisms on the fitness of a particular species.  When an organism is in an adaptive valley, it is in trouble.  There will be selection for the organism to achieve high fitness, perhaps arising through the evolutionary mechanism which achieves a positive instantaneous fitness derivative through the easiest route (it will hit an upward slope the quickest or with the least resistance—although does not have to be the case, high fitness could be achieved when an organism is “knocked around” on the landscape).  The adaptive landscape model also highlights that multiple paths to high fitness are possible, with multiple potential peaks in the landscape. 

 

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