Results of Mac DNAsis analysis
for 1,6 Fructose Bisphosphate Aldolase
 

(This web page was produced as an assignment for an undergraduate course at Davidson College. )

 








Open Reading Frame (ORF)


Figure 1:  Open Reading Frame analysis of the cDNA for the protein 1,6 Fructose Bisphosphate Aldolase in the species Homo sapiens.  Red triangles represent start codons and vertical green lines indicate stop codons.  The blue box highlights the largest open reading frame.  This ORF is from nucleotides x-x and codes for a protein with a molecular weight of 39,417.91 Daltons.
 



Hydrophobicity (Kyte and Doolittle plot)
 

Figure 2:  Kyte and Doolittle Hydrophobicity plot of the Homo sapien 1,6 FBP Aldolase.   There are 4 peaks on the graph which reach a Y-axis value of 2.00 or greater, indicating portions of the protein hydrophobic enough to reside in a phospholipid bilayer.  These points occur near positions 80, 190, 210, and 260.   These data suggests that the protein is an integral membrane protein.
 



Antigenicity (Hopps and Woods plot)
 


Figure 3: Hopps and Woods antigenicity plot for Homo sapien 1,6 FBP Aldolase.  Highly hydrophilic regions (indicated by high Y-axis values) represent possible good epitope sites.  This particular plot suggests that amino acid regions around points 10, 70, 100, 140-150, and 310-330 would be good epitopes to create an antibody for.
 



Predicted Secondary Structure


Figure 4:  Predicted secondary structure of Homo sapiens 1,6 FBP Aldolase using Chou, Fasman, and Rose  analysis.  Click here to compare this prediction to a Rasmol Image of Human 1,6 FBP Aldolase.
 



Multiple Seqeunce Alignnment

Figure 5:  Multiple sequence alignment for 1,6 FBP Aldolase  from 5 different species: Drosophila melanogaster, Homo sapiens, Mus musculus, Saccharomyces cerevisiae, and Schistosoma mansoni.  Amino acids are compared with Black boxes indicating residues common between 2 or more species at a given position along the polypeptide chain.  To see the full amino acid sequence for a species, click on its name.
 



Phylogenetic Tree


Figure 6:  Phylogenetic tree of 1,6 FBP Aldolase from Drosophila melanogaster, Homo sapiens, Mus musculus, Saccharomyces cerevisiae, and Schistosoma mansoni.  MacDNAsis created this phylogenetic tree based on amino acid conservation over time between the different species.   To view the complete amino acid sequence for a species, click on its name.


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