Pyruvate kinase is one of the enzymes involved in glycolysis. Pyruvate kinase catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP:
Phosphoenolpyruvate + ADP + H(+) --> pyruvate + ATP
Figure 1. Chime Image: Pyruvate kinase (dimer) from Saccharomyces
cerevisiae complexed with Pg Mn2+ and K+. (MMDB Id: 11447, PDB Id: 1A3X).
| Organism | Amino acid sequence? | Nucleotide sequence? |
| Homo sapiens | Yes | Yes, mRNA |
| Saccharomyces cerevisiae | Yes | Yes, complete gene (chromosome I) |
| Mus musculus | Yes | Yes, mRNA |
| Arabidopsis thaliana | Yes | Yes, gene (chromosome III) |
| Caenorhabditis elegans | Yes | Yes, gene |