Results of Mac DNAsis for Adenosine Deaminase (ADA)



Open Reading Frame (ORF)

Figure 1.  ORF analysis of Homo sapiens ADA mRNA.  Red triangles indicate start codons, green lines indicate stop codons.  Black box labels largest ORF in the mRNA sequence.  ORF is nucleotides 77-1168.  Click here to view the original mRNA sequence.

This ORF codes for a peptide with 364 amino acid residues and a predicted weight of 40748.26 Daltons.


Predicted Properties of ADA


Hydrophobicity

Figure 2.  Ktye-Doolittle Hydrophobicity Plot for Homo sapiens ADA.  There are no points on this plot above the 2.00 threshold, so no region of the protein is sufficiently hydrophobic to reside in a phospholipid bilayer.  Therefore, ADA is not an integral membrane protein.



Antigenicity

Figure 3.  Hopp and Woods Hydrophilicity Plot for Homo sapiens ADA.   Highly hydrophilic domains likely suitable for use as antibody epitopes occur near residues 130-140, 310, and 340-350.



Secondary Structure

Figure 4.  Secondary structure of Homo sapiens ADA as predicted by Chou, Fasman and Rose analysis.  Click here to compare this prediction to a RasMol image of crystallized ADA.


Interspecific Comparison of ADA Structure


Multiple Sequence Alignment

Figure 5.  Sample of ADA amino acid sequence from human, E. coli, C. elegans, Arabidopsis, and mouse showing conserved residues (highlighted). To see full amino acid sequences for each species, click on its name.



Phylogeny

Figure 6.  Phylogenetic tree for ADA from human, mouse, C. elegans, E. coli, and Arabidopsis.  Phylogeny created from analysis of amino acid sequence conservation.  Numbers indicate percent conservation between species.  To see full amino acid sequences for each species, click on its name.


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