Gene Networks Database

SpGCF1 - SpAN interaction

• REGULATOR: SpGCF1
• TARGET: SpAN
• INTERACTION TYPE: protein-DNA
• INTERACTION RESULT: activation
• TISSUE:
• STAGE: 9-h and 20-h
• TARGET SITE: (AT)(CT)(CT)HNH(AT)CCCCTHHNHN(AT)(AC)N(AT)T(CT)
• PROOFS: DNase I footprinting, EMSA
• REFERENCES: Kozlowski et al., 1996
• COMMENTS: The sequences shared by SpAN protein binding sites III and VI are similar to consensus binding site for SpGCF1. Site III contains a 20/21 identity to the SpGCF1 consensus. It was shown that complexes formed at sites III and VI are supershifted by addition of an antibody raised against recombinant SpGCF1. Control serum does not cause a mobility shift and complexes formed at other SpAN sites are not supershifted by the anti-SpGCF1 antibody.