Gene Networks Database

Hemicentrotus pulcherrimus Genes in Development: Arylsulfatase



In sea urchin embryos arylsulfatase makes up as much as 0.5% of the total protein of the pluteus larva. Arylsulfatase may be a structural component of the extracellular matrices involved in cell movement during morphogenesis (Rapraeger et al., 1981).


A sequence of 551 amino acids was deduced from the arylsulfatase nucleotide sequence. The amino terminal region is rich in hydrophobic amino acids which is characteristic of a signal peptide.
It was estimated that the 21th is the amino terminal of sea urchin arylsulfatase. The molecular mass of the arylsulfatase starting from the 21th glutamine was calculated to be 58827 Da, which is about 4 kDa smaller than that of the subunit (63 kDa) estimated by SDS/polyacrylamide gel electrophoresis (the difference in molecular mass may be due to the glucosylation of the polypeptide).
No protein sequences similar to the deduced arylsulfatase sequence were found (Sasaki et al., 1988).

Subcellular location

In sea urchin embryos arylsulfatase is localized in both the cytoplasm and extracellular matrices (Rapraeger et al., 1981).

Expression Pattern

Nothern blot analysis showed that there are two mRNAs for arylsulfatase, 2.9-kb RNA (major) and a 1.7-kb RNA (minor). The 2.9-kb RNA seems to be a true mRNA for arylsulfatase; the 1.7-kb RNA is probably a specific degradation product of the large mRNA, or it contains a region of close homology to part of the 3` end of the gene.
The unfertilized egg contains a small amount of 2.9-kb mRNA. This amount gradually decreases until the unhatched blastula stage. After hatching, the 2.9-kb mRNA starts to increase drastically. The concentration of arylsulfatase mRNA increases about 2000-fold during development between the 32-cell stage and the prism stage (Sasaki et al., 1988).

mRNA level

Temporal accumulation

Method: Nothern blot analysis
Reference: Sasaki et al., 1988

Unfertilized egg
2-cell stage
32-cell stage
Unhatched blastula
Hatched blastula
Mesenchyme blastula
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Regulatory Regions

Regulatory Connections

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Downstream Genes

Evolutionary Homologues



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