HeWnt-5

Function

HeWnt-5 is a member of the Wnt gene family, one of the principal families of secreted signaling molecules that orchestrate the development of both invertebrate and vertebrete embryos.
Southern blot analysis demonstrated that HeWnt-5 is a single copy gene (Ferkowicz et al., 1998).

Protein

HeWnt-5 is a cell signaling protein.
All idetified sea urchin Wnt sequences contain nine absolutely conserved cysteine residues and a potential N-linked glycosylation sequence (Ferkowicz et al., 1998).
GenBank: 3808208

Subcellular location

Wnt proteins are secreted and do not diffuse more than a few cell diametres from Wnt-expressing cells (Nusse and Varmus, 1992, Siegfried and Perrimon, 1994).

Expression Pattern

The HeWnt-5 transcripts are detected at all stages of development after the early blastula stage. HeWnt-5 transcript levels rise through the late gastrula stage and then exhibit a second phase of increased expression during later stages when the adult rudiment is forming in this species (Ferkowicz et al., 1998).

mRNA level

Temporal accumulation
Method 1: RNase protection assay
Reference: Ferkowicz et al., 1998

Stage	Unfertilized egg	16 cells	Late cleavage	Early blastula	Mesenchyme blastula	Early gastrula	Late gastrula	36-h early larva	53-h midlarva	70-h late larva	94-h metamorphosing juvenile
Level	-	-	-	-	+	+ +	+ +	+	+ +	+ +	+ +