LpC3 gene codes for the cytosceletal type actin (Fang et al., 1996). Actin represents one of the most abundunt and best characterized structural
components in eucaryotic cells, involved in a wide variety of cytoskeletal, locomotive, and tension-generating functions (Davidson, 1989).
Each haploid L. pictus genome contains a single copy of each of the actin genes LpC1, LpC2, LpC3, LpC4, and LpM.
Southern blot analysis of genomic DNA clones have detected no close linkage of L.pictus actin genes in contrast to
the cytosceletal actins of S. purpuratus, which are clustered into two linkage groups of related genes (Fang et al., 1996).
Protein
LpC3 protein belongs to a family of the cytoskeletal
actins (Fang et al., 1996).
SWISS_PROT: Q25379
Subcellular location
Cytoplasmic
Expression Pattern
The LpC3 and LpC4 transcripts showed similar temporal patterns of accumulation and
are much more less abundant than the LpC1 and LpC2 mRNAs.
The transcripts are not detected until the mesenchyme blastula stage.
The level of transcripts increase during gastrulation
and remains constant through the prefeeding pluteus stage.
The LpC3 probe hybridized to two transcripts of different size.
Whole mount in situ hybridization showed little staining in some mesenchyme
cells, presumably secondary, at late gastrula and plutei (Fang et al., 1996).
mRNA level
Temporal accumulation
Method: Nothern blot hybridization
Reference: Fang et al., 1996
Stage
Egg
Mesenchyme blastula
Gastrula
Prism
Pluteus
Level
-
+
+
+
+
Spatial localization
Method: Whole mount in situ hybridization
Reference: Fang et al., 1996
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