Gene Networks Database
Spec2c RSR enhancer
The experiments identify cis-acting transcriptional elements in the 5`flanking
conserved regions of three related Spec genes, Spec1, Spec2a and Spec2c (termed RSR).
If the XhoI fragments of the Spec1, Spec2a and Spec2c contain such
transcriptional enhanser-like elements, these fragments are able to drive
the expression of the CAT gene using the heterologous SV40 promoter.
These fragments were inserted into pSV1 in the forvard and reverse orientation
and injected into L. pictus eggs. Unlike Spec2a, in the case of the Spec2c
XhoI fragment, activity was seen in the forward but not in the reverse orientation.
When the RSR element upstream of the Spec2c gene was aligned with the
corresponding Spec2a element, an insertion of 2942 bp of DNA with respect to
Spec2a was apparent (Gan L. et al., 1990).
Alignment of Spec 5'-flanking DNA
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Comments are welcome to Kate G. Savostyanova
Copyright © 1997 GeNet Team